Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2014, Vol. 36 ›› Issue (4): 389-393.doi: 10.3881/j.issn.1000-503X.2014.04.008

• Original article • Previous Articles     Next Articles

Observing the Effect of High Glucose on Proliferation of Bone Marrow Stromal Stem Cells through Wnt/β-catenin Pathway

ZHANG Bo,LIU Na,GU Bin,WU Hao,GAO Yu-xuan,WANG Dong-sheng,LIU Hong-chen   

  1. Stomatological Center,Chinese PLA General Hospital,Beijing 100853,China
  • Received:2014-01-03 Online:2014-08-31 Published:2014-08-31
  • Contact: LIU Hong-chen Tel:010-66936254,E-mail:liuhongchenpla301@163.com
  • Supported by:
    Supported by the National Natural Sciences Foundation of China (81271738) and National High Technology Research and Development Program of China (2012BAI06B01)

Abstract: Objective To explore the effect of high glucose on proliferation of bone marrow stromal stem cells through Wnt/β-catenin pathway. Methods Bone marrow stormal cells were obtained from the mandible of Wistar rats and stimulated with different concentrations of glucose (5.5 and 16.5 mmol/L). Cell proliferation was evaluated with methyl thiazolyl tetrazolium assay (1,3,5,and 7 d)and cell cycle analysis by flow cytometry (5 d). β-catenin and cyclin D1 protein levels were determined by Western blot. The mRNA expression of lymphoid enhancer binding factor-1 (LEF-1) and cyclin D1 were tested by real-time polymerase chain reaction.Results The results of methyl thiazolyl tetrazolium assay indicated that the optical density values of two different concentrations of the glucose had no statistical difference on day 1 (P=0.700). On days 3,5,and 7,the optical density values of the 16.5 mmol/L group were significantly lower than those in the 5.5 mmol/L group (P=0.006,P=0.002,and P=0.003). Cell cycle analysis indicated that high glucose concentration could reduced the progression from phase G1 to S,and the proliferation index values of the 16.5 mmol/L group were significantly lower than those of the 5.5 mmol/L group (P=0.014). The β-catenin and cyclin D1 levels were lower in the 16.5 mmol/L group when compared with the 5.5 mmol/L group. High glucose condition also reduced the mRNA expressions of LEF-1 and cyclin D1. Conclusion High glucose can inhibit the proliferation of bone marrow stormal cells by suppressing the expressions of β-catenin,LEF-1,and cyclin D1 in the Wnt/β-catenin pathway.

Key words: high glucose, bone marrow stromal stem cells, proliferation, Wnt

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