Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2014, Vol. 36 ›› Issue (4): 394-399.doi: 10.3881/j.issn.1000-503X.2014.04.009

• Original article • Previous Articles     Next Articles

Transient Hepatic Venous Occlusion Induced Liver Hemodynamic Change and Reperfusion Injury in Rats

TAN Xiao-yu1,2,LU Shu-tong1,LIU Jun1,XU Wen-li2,XU Yin-zhe2,GE Xin-lan2,ZHANG Ai-qun2,LI Chong-hui2,DONG Jia-hong2   

  1. 1Department of Hepatobiliary Surgery,General Hospital of Guangzhou Military Command of PLA,Guangzhou 510010,China;2Department of Hepatobiliary Surgery and Hepatobiliary Surgical Institute,Chinese PLA General Hospital,Beijing 100853,China
  • Received:2014-01-06 Online:2014-08-31 Published:2014-08-31
  • Contact: LI Chong-hui Tel:010-66936602,E-mail:lich_ plagh@163.com;DONG Jia-hong Tel:010-66938030,E-mail:dongjh301@163.com
  • Supported by:
    Supported by the National Natural Sciences Foundation of China(81260017) and the Open Project of Key Laboratory in Xinjiang Uygur Autonomous Region (XJYS0906-2011-06)

Abstract: Objective To observe the hemodynamic change and reperfusion injury cause by transient hepatic venous occlusion and transient hepatic inflow occlusion in rats. Methods The rat liver was divided into 3 different areas:the ischemia reperfusion (IR) area:the inflow of the right superior lobe was clamped for half an hour;the non-isolated lobe congestive reperfusion (NIL-CR) area:the outflow of the right median lobe was clamped for half an hour;and the isolated lobe congestive reperfusion (IL-CR) area:the outflow of the left lobe was clamped for half an hour. The flux value and the oxygen saturation of microcirculation were monitored before at clamping for 30 minutes,and on 1 day,3 days,and 7 days after reperfusion. The hepatic damage and Suzuki’s score were evaluated. Results After clamping for 30 minutes,the flux value in the IR area was significantly higher than in NIL-CR area (P<0.01) and IL-CR area (P<0.01),the oxygen saturation in the IR area was significantly higher than in NIL-CR area (P<0.01) and IL-CR area (P<0.05). Compared with IR area,both NIL-CR area and IL-CR area were found having more severe liver damage in terms of Suzuki’s score in early postoperative period (at clamping for 30 minutes and on 1 day,P<0.01). However,there was no significant difference between NIL-CR area and IL-CR area in flux value,oxygen saturation,and Suzuki’s score (P>0.05). Conclusions Hepatic venous occlusion can more effectively decrease the blood perfrusion and oxygen saturation;thus,compared to the IR,CR can result in more severe liver damage. The presence of normal liver tissue around the congestion area can not influence liver damage in transient hepatic venous occlusion.

Key words: hepatic vein, microcirculation, hemodynamics, reperfusion injury

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