Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2014, Vol. 36 ›› Issue (4): 426-431.doi: 10.3881/j.issn.1000-503X.2014.04.014

• Original article • Previous Articles     Next Articles

Role of Vascular Cell Adhesion Molecule-1 in the Mouse Model of Hepatic Ischemia/Reperfusion and the Hematogenic Metastasis

LIU Yi,NING Shang-lei,CHEN Yu-xin,XU Ke-sen,SHOU Nan-hai   

  1. Department of Hepatobiliary Surgery,Qilu Hospital of Shandong University,Jinan 250012,China
  • Received:2013-12-24 Online:2014-08-31 Published:2014-08-31
  • Contact: SHOU Nan-hai Tel:0531-82166651,E-mail:gnileef@163.com
  • Supported by:
    Supported by the National Natural Sciences Foundation of China(81141017)

Abstract: Objective To investigate the effect of ischemia/reperfusion (I/R) on tumor metastasis in a experimental mouse model of hematogenous metastasis after I/R and to quantify expression of vascular cell adhesion molecule-1 (VCAM-1) during I/R. Methods An experimental mouse model of metastasis after partial hepatic I/R was designed to determine the effects of I/R on tumor metastasis to liver. Tumor loads were valued 14 days after operation. In addition,the expressions of alanine transaminase (ALT),aspartate transaminase (AST),and VCAM-1 were detected. Results Two hours after hepatic reperfusion,ALT and AST levels in ischemia 45-minute group and ischemia 30-minute group were significantly higher than in the sham group (all P<0.05). Also,the changes of ALT and AST were more obvious in the ischemia 45-minute group than in ischemia 30-minute group (all P<0.05). In the sham group,both ALT and AST slightly and transiently increased. ALT and AST in the ischemia 45-minute group and ischemia 30-minute group at 8 hours were both significantly higher than those at 2 hours reperfusion (P<0.05). The tumor load (valued by hepatic replacement area) and the expression of VCAM-1 in ischemic lobe were significantly larger in the ischemia 45-minute group than in the ischemia 30-minute group and sham group (P=0.013,P=0.007). However,there was no statistical difference on tumor load between the right lobe of sham operated mice and the right lobe (nonischemic lobes) of mice subjected to I/R (P=0.089). Mouse survivals were significantly longer in the sham group than in the ischemia 30-minute group (P=0.041) but were not significantly different between the ischemia 45-minute group and ischemia 30-minute group (P=0.055). VCAM-1 expression in ischemia 45-minute group was significantly higher than in ischemia 30-minute group and sham group(P=0.003,P<0.001),and it was positively correlated with the hepatic replacement area (r=0.491,P=0.045). Conclusion Hepatic I/R promotes liver hematogenic metastasis of hepatocellular carcinoma in mice and at least in part,through the induction of VCAM-1 expression.

Key words: hepatocellular carcinoma, ischemia/reperfusion, vascular cell adhesion molecule-1, portal vein metastasis

CLC Number: