Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2014, Vol. 36 ›› Issue (5): 477-481.doi: 10.3881/j.issn.1000-503X.2014.05.004

• Original article • Previous Articles     Next Articles

Expression of Calcineurin in Podocytes in Response to Endoplasmic Reticulum Stress in Diabetic Nephropathy

HU Rong-rong1,MA Jie1,TAO Jian-ling1,WEN Yu-bing1,ZHAO Cui2,ZHANG Wei1,LI Hang1,LI Ming-xi1,LI Xue-mei1,LI Xue-wang1   

  1. 1Department of Nephrology,PUMC Hospital,CAMS and PUCM,Beijing 100730,China
    2Department of Nephrology,Cangzhou City People’s Hospital,Cangzhou,Hebei 061000,China
  • Received:2014-07-25 Online:2014-10-31 Published:2014-10-31
  • Contact: TAO Jian-ling Tel:010-69155058,
  • Supported by:
    Supported by the National Natural Sciences Foundation of China(81170665)

Abstract: Objective To investigate the regulation of calcineurin (CaN) by endoplasmic reticulum stress(ERS)in podocytes in vitro and in vivo at the stage microalbuminuria in diabetic nehropathy(DN). Methods The urinary albumin excretions of C57BLKS/JLepr db/db and db/m mice at the ages of 6,9,and 12 weeks were measured. The expressions of CaN and synaptopodin of these mice were observed. In immortalized mouse podocytes,the expression of podocyte CaN incubated with different concentrations of paltimate was quantitatively determined by real-time PCR. The changes of CaN incubated with paltimate with or without ursodeoxy-cholic acid (UDCA) were analyzed by confocal microscopy and Western blotting. Results As urine protein increased,the expression of CaN was enhanced and the expression of synaptopodin was reduced in early stage DN db/db mice potocytes.In immortalized mouse podocytes,as the concentrations of palmitate increased,CaN mRNA increased. By confocal microscopy,the fluorescence intensity of CaN increased in palmitate treatment group.After co-incubation with palmitate and UDCA,the fluorescence intensity decreased. The similar results were shown by Western blotting. Conclusion At the stage of microalbuminuria in DN,ERS in podocytes up-regulates the expression of CaN.

Key words: podocyte, palmitate, ursodeoxycholic acid, calcineurin, synaptopodin, endoplasmic reticulum stress

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