Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2014, Vol. 36 ›› Issue (5): 482-487.doi: 10.3881/j.issn.1000-503X.2014.05.005

• Original article • Previous Articles     Next Articles

Effects of Eukaryotic Translation Initiation Factor 5A2 Down-regulation by Small Interfering RNA on Aggressiveness of MKN28 Human

MENG Qing-bin1,2,YU Jian-chun1,KANG Wei-ming1,MA Zhi-qiang1, ZHOU Li1,YE Xin1,CAO Zhan-jiang1,TIAN Shu-bo1   

  1. 1Department of General Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
    2Department of Gastrointestinal Surgery,the First Hospital of Wuhan,Wuhan 430022,China
  • Received:2014-02-12 Online:2014-10-31 Published:2014-10-31
  • Contact: YU Jian-chun Tel/Fax:010-69152213,E-mail:yu-jch@163.com E-mail:yu-jch@163.com
  • Supported by:
    Supported by the Beijing Municipal Natural Science Foundation(7132209)and the Graduate Innovation Funding from Peking Union Medical College(2011-1002-017)

Abstract: Objective To investigate the effects of eukaryotic translation initiation factor 5A2(EIF5A2)down-regulation by small interfering RNA(siRNA)on aggressiveness of human gastric cancer cell and its potential mechanisms. Methods The expressions of EIF5A2 in human gastric cancer cell lines(MKN28 and HGC27)and immortalized gastric mucosal epithelial cells(GES-1)were measured by real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR)and Western blotting. EIF5A2 gene in MKN28 cells was silenced by RNA interference and the inhibitory effect was evaluated by both qRT-PCR and Western blotting. Cell proliferation was assessed by CCK-8 assay. Cell migration and invasion were assessed by Transwell assay. The possible downstream targets of EIF5A2,such as CyclinD1,CyclinD3,matrix metallopeptidase-9(MMP-9),E-cadherin,vimintin,C-myc,and metastasis-associated protein 1(MTA1)expression levels,were examined by Western blotting. Results High expressions of EIF5A2 were found in MKN28 cells and human gastric adenocarcinoma tissues. Both EIF5A2 mRNA and protein expression in MKN28 cells were significantly down-regulated by siRNA#1 and siRNA#2,especially siRNA#1. Knockdown of EIF5A2 caused an apparent suppression of MKN28 cell proliferation(all P<0.01),migration(P<0.001),and invasion(P<0.001). After the knockdown of EIF5A2 in MKN28 cells,E-cadherin levels were upregulated,whereas vimentin,Cyclin D1,Cyclin D3,C-myc and MTA1 levels were downregulated. Conclusion Knockdown of EIF5A2 may inhibit MKN28 cell proliferation by downregulating the CyclinD1 and CyclinD3 and suppressing the cell migration and invasion by inhibiting MTA1,C-myc and epithelial-mesenchymal transition.

Key words: eukaryotic translation initiation factor 5A2, stomach neoplasms, small interfering RNA, proliferation, metastasis

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