Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2014, Vol. 36 ›› Issue (6): 660-667.doi: 10.3881/j.issn.1000-503X.2014.06.019

• Original Articles • Previous Articles     Next Articles

Effect of Sinusoidal Electromagnetic Field on Bone Mineral Density and Histomorphometry of Rats at Different Time Points

GAO Yu-hai,ZHEN Ping,ZHOU Jian,MA Xiao-ni,YAN Juan-li,LI Shao-feng,ZHOU Yan-feng,CHEN Ke-ming   

  1. Institute of Orthopaedics,Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA,Lanzhou 730050,China
  • Received:2013-12-05 Online:2014-12-30 Published:2014-12-30
  • Contact: CHEN Ke-ming Tel:0931-8994327,

Abstract: Objective To investigate the effect of 50 Hz 0.1 mT sinusoidal electromagnetic field at different time points on bone mineral density(BMD)and histomorphometry in rats. Methods Totally 50 6-week-old female SD rats were equally randomized into 5 groups:control group,45-minute group,90-minute group,180-minute group,and 270-minute group. Except for the control group,the other four groups were given magnetic intervention in the 50-Hz 0.1-mT sinusoidal electromagnetic field for 45 minutes,90 minutes,180 minutes,or 270 minutes,respectively,on a daily basis.After 8 weeks,the total body BMD,femur BMD,and vertebral BMD were measured by dual-energy X-ray absorptiometry. The left tibia and the fifth lumbar vertebrae were separated for bone tissue static and dynamic analyses. Results Compared with control group,the 90-minute group and the 180-minute group had significantly different total body BMD(P<0.01,P<0.05),while no such significant difference was seen in the 45-minute group and 270-minute group (P>0.05). The femur,vertebral BMD,serum biochemical markers,and the static parameters of the fifth lumbar vertebrae tissue showed significant differences in the 90-minute group,180-minute group,and 270-minute group(P<0.01),but not in the 45-minute group (P>0.05). As shown by double fluorescent labeling,the distance was sorted in an order of 90-minute group>180-minutes group>270-minute group>45-minutes group>control group. Conclusion The 50-Hz 0.1-mT sinusoidal electromagnetic field can effectively increase bone mineral density and improve bone morphology;however,the intervention effectiveness differs at different time points,with the best effectiveness seen at 90 minutes.

Key words: sinusoidal electromagnetic fields, bone mineral density, histomorphometry of bone

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