Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2015, Vol. 37 ›› Issue (1): 8-11.doi: 10.3881/j.issn.1000-503X.2015.01.002

• Orginal Article • Previous Articles     Next Articles

Establishment of a Chronic Restraint Stress Model of Depression with C57BL/6J Mice

Nan-nan SUN, Long-ze SHA, Qi XU()   

  1. State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China
  • Received:2014-09-30 Online:2015-02-28 Published:2015-02-12
  • Supported by:
    Supported by the National Natural Sciences Foundation of China(31222031),the Fundamental Research Fund for the Central Universities(2012S05),and the Union Youth Science & Research Fund(2012J09)

Abstract: ObjectiveTo establish a chronic restraint stress(CRS)model of depression,using C57BL/6J mice. MethodsTotally 20 C57BL/6J mice were screened by weight,sucrose preference,and the results of open-field test. Then,they were equally randomized into two groups:normal control(NC)group and CRS group. Mice in the CRS group were under restraint 4 hours a day and their behavioral changes were evaluated after 21 days. ResultsThe immobility time was significantly longer in CRS mice[(131.70±21.65) s] compared with controls[(68.88±8.43) s](P=0.0304). CRS mice showed a significant decrease in sucrose preference during the time 0-24 h [(66.21±3.24)% vs.(79.46±3.85)%,P=0.0196] and 0-48 h [(73.25±1.50)% vs.(80.20±2.26)%,P=0.0248]compared with controls. ConclusionThe C57BL/6J mice CRS models of depression were successfully established.

Key words: depression, chronic restraint stress, C57BL/6J mice, behavioral tests

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