Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2015, Vol. 37 ›› Issue (1): 30-36.doi: 10.3881/j.issn.1000-503X.2015.01.006

• Orginal Article • Previous Articles     Next Articles

Effects of MicroRNA-132 Transfection on the Proliferation and Apoptosis of Human Liver Cancer Cells in vitro and in vivo

Hai-bin LIU(), Ying HUA, Zhou-xiang JIN   

  1. Department of Hepatic Surgery,the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou,Zhejiang 325000,China
  • Received:2014-07-22 Online:2015-06-23 Published:2015-02-12

Abstract: ObjectiveTo observe the biological role and underlying mechanism of microRNA-132 (miR-132) in liver cancer cell proliferation and apoptosis. MethodsThe expressions of miR-132 in the cancer tissue and their adjacent tissues from 45 liver cancer patients were detected by real-time quantitative polymerase chain reaction(RT-qPCR). The biological effects of miR-132 transfection on human liver cancer MHCC97H cells were assessed by CCK-8 assay,flow cytometry,in vivo experiment in nude mice,and TUNEL test. Western blotting was used to detect the expressions of p-AKT,Survivin,and Caspase 3 in liver cancer cells. Immunohistochemistry was used to detect the positive expressions of Ki-67,Survivin,and Caspase 3 in the xenograft tumors. ResultsThe expression level of miR-132 was found to be down-regulated in liver cancer tissues compared with the matched adjacent tissues (P<0.05). After transfection,the expression of miR-132 was significantly higher than blank control group and negative control group(P<0.05). The proliferation of liver cancer cells was inhibited significantly by miR-132 transfection(P<0.05). Transfection of miR-132 arrested cells in the G0/G1 phase and triggered apoptosis of MHCC97H cells(P<0.05). After miR-132 transfection,the expression of Caspase 3 was up-regulated,whereas the expressions of p-AKT and Survivin were down-regulated(P<0.05).In addition,the tumor weight in miR-132 transfection group was significantly decreased in comparison with blank control group and negative control group(P<0.05). Apoptosis occurred more frequently in the miR-132 transfection group than in control groups(P<0.05). Compared with the blank control group and negative control group,the miR-132 transfection group had significantly decreased expression of Survivin but increased positive expression of Ki-67 and Caspase 3(P<0.05). ConclusionsmiR-132 is down-regulated in human liver cancer tissues. miR-132 transfection can effectively inhibit proliferation and promote apoptosis of MHCC97H cells in vitro and in vivo. Therefore,miR-132 may become a new target in liver cancer treatment.

Key words: liver cancer, microRNA-132, apoptosis

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