Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2015, Vol. 37 ›› Issue (1): 37-43.doi: 10.3881/j.issn.1000-503X.2015.01.007

• Orginal Article • Previous Articles     Next Articles

Interaction between the Polymorphisms of Cyclooxygenase-2-1195G/A,MnSOD9Ala/Val Genes and the High-fat Diets and Its Correlation with Ulcerative Colitis

Chao-xian ZHANG1(), Li-ke GUO2, Xiao-feng GUO3   

  1. 1Department of Gastroenterology,
    2Department of Stomatology,the First Affiliated Hospital of Xinxiang Medical University,Weihui,Henan 453100,China
    3Department of Hepatology,the Sixth people’s Hospital of Hangzhou,Hangzhou 310014,China
  • Received:2014-03-12 Online:2015-02-28 Published:2015-02-12

Abstract: ObjectiveTo investigate the interaction of the polymorphisms of cyclooxygenase-2-1195G/A(COX-2-1195G/A)and manganese superoxide dismutase 9Ala/Val(MnSOD9Ala/Val)genes and the high-fat diets and its potential correlation with ulcerative colitis(UC). MethodsThe genetic polymorphisms of COX-2-1195G/A and MnSOD9Ala/Val were analyzed by polymorphism-polymerase chain reaction(PCR)in peripheral blood leukocytes obtained from 750 UC patients(UC group)and 750 healthy subjects(control group). ResultsThe frequencies of COX-2-1195G/A(A/A)and MnSOD9Ala/Val(V/V)were 49.07% and 50.13% in UC group and 21.20% and 22.40% in control group,respectively(P<0.01). The risk of UC significantly increased in subjects with COX-2-1195G/A(A/A)genotype(OR=3.5808,95%CI=1.8062-5.3478)and in those with MnSOD9Ala/Val(V/V)genotype(OR=3.4828,95%CI=1.9137-5.5496). Pooled analysis of the polymorphisms showed that distribution frequency of COX-2-1195G/A(A/A)/MnSOD9Ala/Val(V/V)was 40.67% in UC group and 8.40% in control group(P<0.01). Subjects with COX-2-1195G/A(A/A)/MnSOD9Ala/Val(V/V)had a significantly higher risk of UC(OR=7.5655,95%CI=4.1849-11.2037). The rate of high-fat diets was significantly higher in the UC group than in the control group(49.73 vs.20.13%,P<0.01),and statistic analysis suggested an interaction between high-fat diet and COX-2-1195G/A(A/A)(γ=11.81821)and MnSOD9Ala/Val(V/V)(γ=9.0107),which increase risk of UC. ConclusionsCOX-2-1195G/A(A/A),MnSOD9Ala/Val(V/V),and high-fat diet are the risk factors of UC. The interaction between the genetic polymorphisms of COX-2-1195G/A and MnSOD9Ala/Val and the high-fat diet increases the risk of UC.

Key words: ulcerative colitis, cyclooxygenase-2-1195G/A, manganese superoxide dismutase 9Ala/Val, polymorphism, high-fat diet

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