环氧合酶-2-1195G/A和锰超氧化物歧化酶9Ala/Val基因多态性与高脂饮食的交互作用及其与溃疡性结肠炎的关系

doi:10.3881/j.issn.1000-503X.2015.01.007

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2015, Vol. 37 ›› Issue (1): 37-43.doi: 10.3881/j.issn.1000-503X.2015.01.007

• Orginal Article • Previous Articles Next Articles

Interaction between the Polymorphisms of Cyclooxygenase-2-1195G/A,MnSOD9Ala/Val Genes and the High-fat Diets and Its Correlation with Ulcerative Colitis

Chao-xian ZHANG¹(), Li-ke GUO², Xiao-feng GUO³

¹Department of Gastroenterology,
²Department of Stomatology,the First Affiliated Hospital of Xinxiang Medical University,Weihui,Henan 453100,China
³Department of Hepatology,the Sixth people’s Hospital of Hangzhou,Hangzhou 310014,China

Received:2014-03-12 Online:2015-02-28 Published:2015-02-12

Abstract

Abstract: ObjectiveTo investigate the interaction of the polymorphisms of cyclooxygenase-2-1195G/A(COX-2-1195G/A)and manganese superoxide dismutase 9Ala/Val(MnSOD9Ala/Val)genes and the high-fat diets and its potential correlation with ulcerative colitis(UC). MethodsThe genetic polymorphisms of COX-2-1195G/A and MnSOD9Ala/Val were analyzed by polymorphism-polymerase chain reaction(PCR)in peripheral blood leukocytes obtained from 750 UC patients(UC group)and 750 healthy subjects(control group). ResultsThe frequencies of COX-2-1195G/A(A/A)and MnSOD9Ala/Val(V/V)were 49.07% and 50.13% in UC group and 21.20% and 22.40% in control group,respectively(P<0.01). The risk of UC significantly increased in subjects with COX-2-1195G/A(A/A)genotype(OR=3.5808,95%CI=1.8062-5.3478)and in those with MnSOD9Ala/Val(V/V)genotype(OR=3.4828,95%CI=1.9137-5.5496). Pooled analysis of the polymorphisms showed that distribution frequency of COX-2-1195G/A(A/A)/MnSOD9Ala/Val(V/V)was 40.67% in UC group and 8.40% in control group(P<0.01). Subjects with COX-2-1195G/A(A/A)/MnSOD9Ala/Val(V/V)had a significantly higher risk of UC(OR=7.5655,95%CI=4.1849-11.2037). The rate of high-fat diets was significantly higher in the UC group than in the control group(49.73 vs.20.13%,P<0.01),and statistic analysis suggested an interaction between high-fat diet and COX-2-1195G/A(A/A)(γ=11.81821)and MnSOD9Ala/Val(V/V)(γ=9.0107),which increase risk of UC. ConclusionsCOX-2-1195G/A(A/A),MnSOD9Ala/Val(V/V),and high-fat diet are the risk factors of UC. The interaction between the genetic polymorphisms of COX-2-1195G/A and MnSOD9Ala/Val and the high-fat diet increases the risk of UC.

Key words: ulcerative colitis, cyclooxygenase-2-1195G/A, manganese superoxide dismutase 9Ala/Val, polymorphism, high-fat diet

CLC Number:

R574.62

Chao-xian ZHANG, Li-ke GUO, Xiao-feng GUO. Interaction between the Polymorphisms of Cyclooxygenase-2-1195G/A,MnSOD9Ala/Val Genes and the High-fat Diets and Its Correlation with Ulcerative Colitis[J].Acta Academiae Medicinae Sinica, 2015, 37(1): 37-43.

Figures/Tables 3

Table 1

Interaction of the COX-2-1195G/A and MnSOD9Ala/Val genes polymorphisms in UC"

分组 Group	COX-2-1195G/A (GG+GA)+ MnSOD9Ala/Val (AA+AV)	COX-2-1195G/A (GG+GA)+ MnSOD9Ala/Val (VV)	COX-2-1195G/A (AA)+ MnSOD9Ala/Val (AA+AV)	COX-2-1195G/A (AA)+ MnSOD9Ala/Val (VV)
对照 Control	486(64.80%)	105(14.00%)	96(12.80%)	63(8.40%)
UC	311(41.47%)	71(9.47%)	63(8.40%)	305(40.67%)
OR^a	1.0000	1.0567^b(OR₁)	1.0255^c(OR₂)	7.5655^d(O $R 1 * 2$ )
95%CI		0.6724-2.2871	0.6598-1.9925	4.1849-11.2037
β		0.3647(β₁)	0.3539(β₂)	2.6115(β_12*)

Table 1

Table 2

Table 3

References 20

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分组 Group	COX-2-1195G/A(GG+GA)+ 低脂饮食 Low-fat diets	COX-2-1195G/A(GG+GA)+ 高脂饮食 High-fat diets	COX-2-1195G/A(AA)+ 低脂饮食 Low-fat diets	COX-2-1195G/A(AA)+ 高脂饮食 High-fat diets
对照 Control	481(64.13%)	110(14.67%)	118(15.73%)	41(5.47%)
UC	313(41.73%)	69(9.20%)	64(8.53%)	304(40.53%)
OR^a	1.0000	0.9640^b	0.8335^c	11.3944^d
95%CI		0.5913-1.8692	0.6328-1.8407	5.7291-15.4827
β		0.3328(β_e)	0.2897(β_g)	3.9331(β_e_g)

分组 Group	MnSOD9Ala/Val(AA+AV)+ 低脂饮食 Low-fat diets	MnSOD9Ala/Val(AA+AV)+ 高脂饮食 High-fat diets	MnSOD9Ala/Val(VV)+ 低脂饮食 Low-fat diets	MnSOD9Ala/Val(VV)+ 高脂饮食High-fat diets
对照 Control	483(64.40%)	99(13.20%)	116(15.47%)	52(6.93%)
UC	309(41.20%)	65(8.67%)	68(9.07%)	308(41.07%)
OR^a	1.0000	1.0263^b	0.9163^c	9.2584^d
95%CI		0.6917-1.9704	0.6493-1.8713	5.6108-14.5838
β		0.3551(β_e)	0.3170(β_g)	3.1997(β_eg)

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Interaction between the Polymorphisms of Cyclooxygenase-2-1195G/A,MnSOD9Ala/Val Genes and the High-fat Diets and Its Correlation with Ulcerative Colitis

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