Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2015, Vol. 37 ›› Issue (2): 152-156.doi: 10.3881/j.issn.1000-503X.2015.02.004

• Orginal Article • Previous Articles     Next Articles

Establishment and Assessment of Rat Models of Glucocorticoid-induced Osteonecrosis

Yu-lei DONG, Lei ZHOU, Yu-long LI, Ke XIAO, Xi-sheng WENG()   

  1. Department of Orthopedics,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
  • Received:2014-12-01 Online:2015-02-28 Published:2015-05-01
  • Supported by:
    国Supported by the National Natural Sciences Foundation of China(81272009)

Abstract: ObjectiveTo establish the rat models of glucocorticoid-induced osteonecrosis and evaluate the osteonecrosis by high-resolution micro-CT and histomorphology.MethodsTotally 30 Sprague-Dawley male adult rats(age:12 weeks;body weight:400-450 g)were randomized into two groups:one group received two doses of intraperitoneal injection of lipopolysaccharide(20 μg/kg)at an interval of 24 hours,24 hours later,the rats received three doses of intramuscular injection of methylprednisolone(40 mg/kg)at an interval of 24 hours;in the control group,the rats received same amount of normal saline.Four weeks later,5 rats in each group were sacrificed randomly.Eight weeks later,all the remaining rats were sacrificed and the femur specimens were scanned by micro-CT.The trabeculae parameters of the femoral head were calculated.The hematoxylin and eosin staining were performed to calculated the success rate.ResultsThe incidence of osteonecrosis was 80% in the experimental group.The micro-CT demonstrated broken and cystic degeneration.The quantitative analysis showed that the bone volume/total volume(experimental group:0.55±0.13 vs. control group: 0.68±0.12;P<0.05),bone surface area / bone volume(experimental group:20.45±5.13 vs. control group: 24.00±5.20;P<0.05),and trabeculae number(experimental group: 5.67±0.50 vs. control group: 6.24±0.96;P<0.05)were significantly lower in experimental group than those in control group.ConclusionsA rat model of glucocorticoid-induced osteonecrosis was successfully established by lipopolysaccharide and methylpredniso- lone.High-resolution micro-CT is useful for the qualitative and quantitative analyses of the morphology and structure of trabeculae,showing good consistence with the histomorphological findings.

Key words: glucocorticoids, osteonecrosis, micro-CT

CLC Number: