Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2015, Vol. 37 ›› Issue (4): 440-445.doi: 10.3881/j.issn.1000-503X.2015.04.013

• Orginal Article • Previous Articles     Next Articles

Effect of Intestinal Ischemia-reperfusion Injury on the Expression of Chemerin in Mice

Qian-kun ZHU, Gui-zhen HE(), Hai-long LI   

  1. Department of Parenteral and Enteral Nutrition,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
  • Received:2015-02-09 Online:2015-05-25 Published:2015-08-31

Abstract: Objective

To investigate the effect of intestinal ischemia-reperfusion on the protein expression of chemerin in C57BL/6J mice.

Methods

A total of 16 mice(C57BL/6J,specific pathogen free level)were randomly assigned into two groups(n=8 each):the intestinal ischemia-reperfusion group and the sham group. The intestinal ischemia-reperfusion state was achieved by blocking the super-mesenteric artery. After 60 minutes of ischemia followed by 60 minutes of reperfusion,we determined the protein level of chemerin in various organs and tissues by enzyme-linked immunosorbent assay and Western blot. The sham group underwent the same operation process except for the blocking of the super-mesenteric artery.

Result

The protein level of chemerin was significantly elevated in distinctive organs and tissues in the state of intestinal ischemia-reperfusion(P<0.05).

Conclusion

The intestinal ischemia-reperfusion can remarkably increase the protein expression of chemerin in some organs and tissues.

Key words: intestinal ischemia-reperfusion, chemerin, adipocytokine, inflammation, oxidative damage

CLC Number: