Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2015, Vol. 37 ›› Issue (5): 489-495.doi: 10.3881/j.issn.1000-503X.2015.05.001

• Orginal Article •     Next Articles

Dynamic Changes of the Quantitative Distribution,Apoptosis and Proliferation of T and B Cells in the Skin of KM Mutant Mice

Yan-hong LI1, Ying LIU1, Lan HUANG1, Yan-feng XU1, Hua ZHU1, Tao LI2, Wei DENG1, Chuan QIN1()   

  1. 1Key Laboratory of Human Diseases Animal Models,State Administration of Traditional Chinese Medicine, Key Laboratory of Human Diseases Comparative Medicine of Ministry of Health, Institute of Medical Laboratory Animal Science,CAMS and PUMC,Beijing 100021,China
    2Peking Union Medicine College Clinics,CAMS and PUMC,Beijing 100730,China
  • Received:2014-10-18 Online:2015-10-15 Published:2015-10-31
  • Supported by:
    Supported by the National Natural Sciences Foundation for Young Scholars of China(31301928)


Objective To observe the change of quantitative distribution,apoptosis and proliferation of T and B cells in the skin of KM mutant mice. Methods We chose 1-,3-,6-,9-,22-day,3-,6-month-old KM mutant and wild-type mice to detect the changes of T and B lymphocytes using blood routine tests and immunohistochemical staining. Apoptosis was detected by TUNEL staining and proliferation by proliferating cell nuclear antigen (PCNA) staining. Results T cells on KM mutant mice skin were mainly seen in epidermis and dermis. They increased on the first day to 6th day after birth and decreased on the 9th and 22nd day,but after 3-month-old,their number began to increase;at the time of 6 months,the number of B cells also increased. The apoptosis of the skin hair follicle and sebaceous gland cells were more obvious in KM mutant mice than in wild-type mice,with the maximal apoptosis occurred at the age of 22-day-old in both groups. The proliferation of epidermal basal cells in KM mutant mice between 1 to 9-day-old was not significantly different from that in the wild-type mice,but decreasing on the 22nd day and 3rd month and increasing in the 6th month. The proliferation in hair follicle and sebaceous glands decreased on 9th day,increased on 22nd day,and deceased on the 3rd month again. Conclusions The quantitative distribution,apoptosis,and proliferation of T and B lymphocytes abnormally change in the skin tissue of KM spontaneous mutant mice. They may lead to immune and hair growth disorders and promote the inflammatory responses.

Key words: KM mice, spontaneous mutation, T cell, B cell, apoptosis, proliferation

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