KM突变小鼠皮肤T、B细胞数量分布和皮肤细胞凋亡增殖的动态改变

doi:10.3881/j.issn.1000-503X.2015.05.001

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2015, Vol. 37 ›› Issue (5): 489-495.doi: 10.3881/j.issn.1000-503X.2015.05.001

• Orginal Article • Next Articles

Dynamic Changes of the Quantitative Distribution,Apoptosis and Proliferation of T and B Cells in the Skin of KM Mutant Mice

Yan-hong LI¹, Ying LIU¹, Lan HUANG¹, Yan-feng XU¹, Hua ZHU¹, Tao LI², Wei DENG¹, Chuan QIN¹()

¹Key Laboratory of Human Diseases Animal Models,State Administration of Traditional Chinese Medicine, Key Laboratory of Human Diseases Comparative Medicine of Ministry of Health, Institute of Medical Laboratory Animal Science,CAMS and PUMC,Beijing 100021,China
²Peking Union Medicine College Clinics,CAMS and PUMC,Beijing 100730,China

Received:2014-10-18 Online:2015-10-15 Published:2015-10-31
Supported by:
Supported by the National Natural Sciences Foundation for Young Scholars of China(31301928)

Abstract

Abstract:

Objective To observe the change of quantitative distribution,apoptosis and proliferation of T and B cells in the skin of KM mutant mice. Methods We chose 1-,3-,6-,9-,22-day,3-,6-month-old KM mutant and wild-type mice to detect the changes of T and B lymphocytes using blood routine tests and immunohistochemical staining. Apoptosis was detected by TUNEL staining and proliferation by proliferating cell nuclear antigen (PCNA) staining. Results T cells on KM mutant mice skin were mainly seen in epidermis and dermis. They increased on the first day to 6^th day after birth and decreased on the 9^th and 22^nd day,but after 3-month-old,their number began to increase;at the time of 6 months,the number of B cells also increased. The apoptosis of the skin hair follicle and sebaceous gland cells were more obvious in KM mutant mice than in wild-type mice,with the maximal apoptosis occurred at the age of 22-day-old in both groups. The proliferation of epidermal basal cells in KM mutant mice between 1 to 9-day-old was not significantly different from that in the wild-type mice,but decreasing on the 22^nd day and 3^rd month and increasing in the 6^th month. The proliferation in hair follicle and sebaceous glands decreased on 9^th day,increased on 22^nd day,and deceased on the 3^rd month again. Conclusions The quantitative distribution,apoptosis,and proliferation of T and B lymphocytes abnormally change in the skin tissue of KM spontaneous mutant mice. They may lead to immune and hair growth disorders and promote the inflammatory responses.

Key words: KM mice, spontaneous mutation, T cell, B cell, apoptosis, proliferation

CLC Number:

R392.3

Yan-hong LI, Ying LIU, Lan HUANG, Yan-feng XU, Hua ZHU, Tao LI, Wei DENG, Chuan QIN. Dynamic Changes of the Quantitative Distribution,Apoptosis and Proliferation of T and B Cells in the Skin of KM Mutant Mice[J].Acta Academiae Medicinae Sinica, 2015, 37(5): 489-495.

Figures/Tables 8

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References 18

[1]	李彦红,刘颖,黄澜,等. KM突变小鼠慢性炎症性皮肤病的免疫学改变[J].中国实验动物学报,2012,20(2):54-58.
[2]	Peters J,Selley RL,Cocking Y.Mouse gene list[J]. Mouse Genome,1997,95(2):193-466.
[3]	Mayer TC,Kleiman NJ,Green MC.Depilated,a mutant gene that affects the coat of the mouse and acts in the epidermis[J]. Genetics,1976,84(1):59-65.
[4]	徐宏彬. 无毛小鼠及其在皮肤病学研究中的应用[J].上海实验动物科学,1993,13(2):118-121.
[5]	Adkins B,Leclerc C,Marshall-Clarke S.Neonatal adaptive immunity comes of age[J]. Nat Rev Immunol,2004,4(7):553-564.
[6]	Schallert N,Pihlgren M,Kovarik J,et al.Generation of adult-like antibody avidity profiles after early-life immunization with protein vaccines[J]. Eur J Immunol,2002,32(3):752-760.
[7]	Giorgetti CA,Press JL.Somatic mutation in the neonatal mouse[J]. J Immunol,1998,161(11):6093-6104.
[8]	Panteleyev AA,Paus R,Ahmad W,et al.Molecular and functional aspects of the hairless (hr) gene in laboratory rodents and humans[J]. Exp Dermatol,1998,7(5):249-267.
[9]	Paus R,Handjiski B,Czarnetzki BM,et al.A marine model for inducing andmanipulating hair follicle regression (cat agen):effects of dexamethasone and cyclosporine[J]. J Invest Dermatol,1994,103(2):143-147.
[10]	Stenn K,Parimoo S,Prouty S.Growth of the hair follicle:a cycling and regenerating biological system[M]//Chuong CM,ed. Molecular basis of epithelial appendage morphogenesis. Austin,USA:R. G.Landes Co,1998:111-131.
[11]	Paus R,Cotsarelis G.The biology of hair follicles[J]. N Engl J Med,1999,341(7):491-497.
[12]	Stenn KS,Paus R.Control of hair follicle cycling[J]. Physiol Rev,2001,81(1):449-494.
[13]	雷明星. GasderminA3在小鼠毛囊生长期向退化期转换过程中的作用及机制研究[D];重庆:重庆大学,2010:3-5.
[14]	Voskas D,Babichev Y,Ling LS,et al.An eosinophil immune response characterizes the inflammatory skin disease observed in Tie-2 transgenic mice[J]. J Leukoc Biol,2008,84(1):59-67.
[15]	Finck BK,Chan B,Wofsy D.Interleukin 6 promotes murine lupus in NZB/NZW F1 mice[J]. J Clin Invest,1994,94(2):585-591.
[16]	Emlen W,Niebur J,Kadera R.Accelerated in vitro apoptosis of lymphocytes from patients with systemic lupus erythematosus[J]. J Immunol,1994,152(7):3685-3692.
[17]	Liang YH.Chronic proliferative dermatitis in mice:NFκB activation autoinflammatory disease[J]. Patholog Res Int,2011:1-9.
[18]	张敏,朱晓文,王雪儿,等.C57BL/6J小鼠皮肤毛囊发育的实验研究[J].中国临床解剖学杂志,2010,28(1):74-77.

分组Group	1 d	3 d	6 d	9 d	22 d	3个月 3 months	6个月 6 months
CD3⁺T淋巴细胞 CD3⁺T lymphocyte cell
野生小鼠Wild-type mice	4.67±1.68	14.00±2.70	5.78±1.13	22.00±6.19	19.22±4.17	7.45± 2.38	14.13±2.26
突变小鼠Mutant mice	15.67±4.17^a	38.12±5.16^a	10.67±2.19^a	12.57±5.25^a	12.11±2.08^b	42.85±11.74^ac	22.33±4.85^b
CD79a⁺B淋巴细胞 CD79a⁺B lymphocyte cell
野生小鼠Wild-type mice	16.37±1.59	3.65±1.03	10.75±2.14	12.13±3.72	15.86±5.14	9.30±4.02	14.22±5.58
突变小鼠Mutant mice	18.25±3.19	5.75±1.05	9.13±1.18	15.25±6.11	18.75±4.26	11.64±4.29	22.44±5.05^ad

分组Group	1 d	3 d	6 d	9 d	22 d	3个月3 months	6个月6 months
野生小鼠Wild-type mice	7.00±2.11	7.83±3.80	6.00±2.85	5.00±1.78	24.85±7.98^f	4.91±2.78	10.38±4.36
突变小鼠Mutant mice	7.67±1.65	10.67±2.53^a	10.33±2.10^a	9.01±4.82	28.86±10.22^c	14.58±4.85^bd	21.26±6.51^bce

分组Group	1 d	3 d	6 d	9 d	22 d	3个月3 months	6个月6 months
表皮Epidermis
野生小鼠Wild-type mice	23.76± 4.14	31.23± 7.08	28.67± 7.26	24.78± 8.07	52.8±10.32	54.67±11.55	64.29±12.36
突变小鼠Mutant mice	24.35± 3.26	28.67± 4.17	36.26± 5.24	17.58± 8.29	13.06± 5.92^a	10.79± 5.94^a	100.61±27.55^a
毛囊/皮脂腺 Hair follicle/sebaceous gland
野生小鼠Wild-type mice	123.38±10.14	149.14±12.16	124.43±16.75	285.00±23.14	53.89±25.21	336.25±63.29	98.23±25.38
突变小鼠Mutant mice	128.75±43.88	144.43±9.63	110.5±10.42	219.08±11.13^a	221.0±44.15^a	165.54±28.31^a	108.56±62.21

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Dynamic Changes of the Quantitative Distribution,Apoptosis and Proliferation of T and B Cells in the Skin of KM Mutant Mice

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