羧胺三唑对大鼠佐剂性关节炎的治疗作用

doi:10.3881/j.issn.1000-503X.2016.01.009

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2016, Vol. 38 ›› Issue (1): 49-54.doi: 10.3881/j.issn.1000-503X.2016.01.009

• Orginal Article • Previous Articles Next Articles

Therapeutic Effect of Carboxyamidotriazole on Adjuvant Arthritis in Rats

Lei ZHU, Juan LI, Lei GUO, Xiao-li YU, Dan-wei WU, Wei CHEN, Chen CHEN, Xiao-wan DU, De-chang ZHANG(), Cai-ying YE()

Department of Pharmacology,Institute of Basic Medical Sciences,CAMS and PUMC, Beijing 100005,China

Received:2015-03-25 Online:2016-01-15 Published:2016-03-02
Supported by:
Supported by the National Natural Sciences Foundation of China (81102454,81201728,81402943) and the Special Program for Key Basic Research of the Ministry of Science and Technology of China (2014ZX09507003-003)

Abstract

Abstract:

Objective To study the effect of carboxyamidotriazole (CAI) on adjuvant arthritis (AA) in rats.Methods The rats were randomly divided into normal group,two vehicle groups (polyethylene glycol 400 control and normal sodium control group),CAI-treated groups (10,20,and 40 mg/kg) and positive control dexamethasone group. Freund’s completed adjuvant was used to induce AA in rats. The arthritis index (AI) was scored,and X-ray check of the hind limbs and histopathological examination were performed. The levels of tumor necrosis factor (TNF)-α,interleukin (IL)-1β,and IL-6 in the inflamed paw tissues were measured.Results The administration of CAI significantly decreased the AI,restored the body weights,and ameliorated the radiological and histopathological features of joint destruction in AA rats (P<0.05,P<0.01). In addition,CAI reduced the TNF-α,IL-1β,and IL-6 levels in the inflamed paw tissues (P<0.05,P<0.01).Conclusion CAI has therapeutic effect on AA rats,which may be achieved by decreasing the pro-inflammatory cytokines at the site of inflammation.

Key words: carboxyamidotriazole, adjuvant arthritis, cytokines

CLC Number:

R961

Lei ZHU, Juan LI, Lei GUO, Xiao-li YU, Dan-wei WU, Wei CHEN, Chen CHEN, Xiao-wan DU, De-chang ZHANG, Cai-ying YE. Therapeutic Effect of Carboxyamidotriazole on Adjuvant Arthritis in Rats[J].Acta Academiae Medicinae Sinica, 2016, 38(1): 49-54.

Figures/Tables 6

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References 14

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分组 Group	放射学分数 Radiological scores			病理学分数 Histological scores
分组 Group	骨质疏松Osteoporosis		关节间隙狭窄Joint space narrowing	骨侵蚀Bone erosion
NC	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00
PEG 400	5.29±0.16^a	5.36±0.20^a	5.29±0.22^a	3.75±0.25^a
NS	5.21±0.21^a	5.07±0.20^a	5.14±0.27^a	3.75±0.25^a
CAI 低剂量 CAI low-dose	4.43±0.23	4.50±0.25	4.57±0.40	3.25±0.48
CAI 中剂量 CAI middle-dose	4.29±0.19^b	3.86±0.27^c	4.07±0.16^b	2.00±0.41^b
CAI 高剂量 CAI high-dose	2.86±0.18^c	3.57±0.27^c	3.29±0.19^c	1.75±0.25^c
Dex	4.50±0.17	4.07±0.20^b	1.57±0.20^c	1.25±0.25^c

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