Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2016, Vol. 38 ›› Issue (2): 198-204.doi: 10.3881/j.issn.1000-503X.2016.02.013

• Orginal Article • Previous Articles     Next Articles

Construction of Nude Mouse Model of Triple Negative Breast Cancer and Value of Thermal Tomography

Hui-ping HUO1, Jian-bin HUO2, Wen-bo WAN1, Jun-lai LI1()   

  1. 1 Department of South Building Ultrasound,Chinese PLA General Hospital,Beijing 100853,China
    2 Department of Health,Inner Mongolia Bayinnaoer City Linhe People’s Hospital,Bayannur,Inner Mongolia 015000,China;
  • Received:2015-12-11 Online:2016-02-15 Published:2016-04-10


Objective To explore the early detection of breast cancer by ultrasonic imaging and thermal tomography of luciferase or green fluorescent protein (GFP)-labeled MDA-MB-231 breast cancer cell line-xenografts in nude mice. Methods Fluorescence-tagged lentiviral vectors were transfected into the triple-negative breast cancer cell line MDA-MB-231. These cells were implanted either subcutaneously under the right breast pad or intravenously into the tail vein of nude BALB/C mice. Thermal tomography and ultrasound imaging were used to detect tumor formation and to monitor tumor growth and metastasis in vivo.Results Triple negative breast cancer cell line-xenografts were used to successfully construct an orthotopic nude mice model of breast cancer metastasis in the peritoneum. Thermal tomography and ultrasound imaging were used together to detect small tumors. Thermal tomography imaging detected small tumors earlier than ultrasound imaging. Conclusions Thermal tomography can be used to monitor changes in tumor growth and detect abnormal tissue. Therefore,it can serve as a convenient,rapid,sensitive,and reliable technique for early screening of human breast cancer.

Key words: triple negative breast cancer, animal model, thermal tomography imaging system, in vivo imaging spectrum system

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