Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2016, Vol. 38 ›› Issue (2): 217-221.doi: 10.3881/j.issn.1000-503X.2016.02.016

• Orginal Article • Previous Articles     Next Articles

Impacts of Erythropoietin on Vascular Endothelial Growth Factor Receptor 2 by the Extracellular Signal-regulated Kinase Signaling Pathway in a Neonatal Rat Model of Periventricular White Matter Damage

Qi-chao YUAN, Li JIANG(), Li-hua ZHU, Da-fan YU   

  1. Department of Pediatrics,Zhongda Hospital,Medicine School,Southeast University,Nanjing 210009,China
  • Received:2015-10-10 Online:2016-02-15 Published:2016-04-10

Abstract:

Objective To explore the impacts of erythropoietin on vascular endothelial growth factor receptor 2 (VEGFR2) by the extracellular signal-regulated kinase (ERK) signaling pathway in a neonatal rat model of periventricular white matter damage. Methods All of postnatal day 4 rats were randomized into three groups:the sham group [without hypoxia-ischemia (HI)],the HI group (HI with saline administration),and the erythropoietin(EPO) group [HI with recombinant human erythropoietin(rh-EPO) administration]. Rat pups underwent permanent ligation of the right common carotid artery,followed by 6% O2 for 2 hours or sham operation and normoxic exposure. Immediately after the HI,rats received a single intraventricular injection of rh-EPO (0.6 IU/g body mass) or saline. ERK and phosphorylation-ERK were examined at 60 minutes and 90 minutes after operation,and VEGFR2 were detected at 2 and 4 days after operation by using Western blot. Results At 60 minutes and 90 minutes after operation,the proteins of phosphorylation-ERK were significantly higher in HI rats than in the sham rats and significantly higher in HI+EPO rats than in the HI rats (P<0.05). Two days after operation,VEGFR2 was not significantly different between sham and HI rats. However,the proteins of VEGFR2 were increased after administration of rh-EPO(P<0.05). Four days after operation,the proteins of VEGFR2 were significantly higher in HI rats than in the sham rats and significantly higher in HI+EPO rats than in the HI rats(P<0.05).Conclusion EPO may regulate VEGFR2 expression by affecting the intracranial ERK signaling pathways.

Key words: erythropoietin, periventricular white matter damage, extracellular signal-regulated kinase, vascular endothelial growth factor receptor 2

CLC Number: