Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2016, Vol. 38 ›› Issue (3): 253-259.doi: 10.3881/j.issn.1000-503X.2016.03.002

• Orginal Article • Previous Articles     Next Articles

Effect of Endometrial Stem Cell-derived Cytokine Cocktail on a Mouse Model of Myocardial Reperfusion Injury

Zhi JIANG1, Zhong-shen JIA2, Yu-jia PAN1,2, An-jie LI1, Fang WEI1()   

  1. 1Department of Cardiology,Guizhou Provincial People’s Hospital,Guizhou 550002,China;
    2Department of Clinical Medicine,Guizhou Medical University,Guizhou 550025,China
  • Received:2015-12-25 Online:2016-03-15 Published:2016-06-10

Abstract:

Objective To study the effect of endometrial stem cells (EnSCs) derived cytokine cocktail (EdCC) on myocardial ischemic reperfusion injury (I/R) in a mouse model. Methods EdCC was concentrated from the culture medium of EnSCs with Millipore ultra-filtration technology and was administrated to a myocardial I/R mouse models through tail vein injection. The infarct area was determined by TTC/Evans Blue staining. The apoptotic cells were counted by TUNEL assay and the protein level of cleaved caspase3 was evaluated by Western blotting. Results The EdCC extraction efficiency was (222.4±29.3) μg/106 cells in every 24 h,but the protein gradually degraded under-80 ℃ storage. As compared with I/R group,100 μg fresh EdCC decreased infarct area (P=0.001),reduced apoptotic nuclei in the infarct border (P=0.019),and inhibited cleaved caspase 3 expression (P=0.002). Increasing EdCC dosage did not further reduce the infarct area. The myocardial protective effect of EdCC diminished after 90 days’ storage under-80 ℃. Conclusion EdCC reduces myocardial I/R injury through protecting cardiomyocytes from apoptosis within 30 days storage under-80 ℃.

Key words: endometrial stem cells, cytokine, myocardial infarction, ischemic reperfusion injury, apoptosis

CLC Number: