Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2017, Vol. 39 ›› Issue (2): 240-246.doi: 10.3881/j.issn.1000-503X.2017.02.013

• Orginal Article • Previous Articles     Next Articles

Preliminary Analysis of Parkinson-like Motor Coordination Abnormityin Brain-specific hS100B Transgenic Mice

Jialin LIU1, Fang ZHENG2, Yan LONG2, Liu TONG2, Yuan ZHENG2, Xiaoqing LIU2, Chuan QIN3()   

  1. 1Department of Pathology,Hebei University Medical College,Baoding,Hebei 071000,China
    2Department of Geratology,the First Central Hospital of Baoding,Baoding,Hebei 071000,China
    3Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences,Comparative Medicine Center,Peking Union Medical College,Beijing 100021,China
  • Received:2016-01-04 Online:2017-04-20 Published:2017-04-20
  • Supported by:
    Supported by Baoding Sciences and Technology Research and Development Program (14ZF003),Development Fund for Hebei University Medical Science (2014A1002),and Research Fund for the Doctoral Program of Hebei University (Special Funds for A Provincial University)

Abstract:

Objective To investigate the role of S100B in the development of Parkinson’s disease (PD) and explore the possibility of brain-specific S100B transgenic mice as PD animal model. Methods The hS100B transgenic mice were established. The mice were divided into S100B transgenic group (TG),S100B knockout group (KG),and the non-transgenic control group (CG). Motor coordination ability of mice was measured by the rota-rod and pole-climbing test. The expressions of S100B,dopamine D1 receptor,dopamine D2 receptor,G protein-coupled receptor kinase (GRK)2,GRK5,and tyrosine hydroxylase in brain tissue were detected by reverse transcription-polymerase chain reaction and Western blot. The levels of tyrosine,levodopa,dopamine,and homovanillic acid in brain tissue were measured by high-performance liquid chromatography coupled with fluorescence detection. Results Compared with CG,the S100B protein expression in brain tissue significantly increased in TG (P<0.05);the motor coordination ability of mice showed progressive decline (P<0.05);the mRNA and protein expressions of dopamine D2 receptor and GRK2 significantly decreased (P<0.05);the levels of levodopa,dopamine,and homovanillic acid were significantly elevated (P<0.05);the expression of tyrosine hydroxylase was also down-regulated,although there was no significant difference (P>0.05). Compared with CG,there was no obvious change of the above indicators in KG (all P>0.05). Conclusion S100B plays an important role in the motor coordination abnormity of PD. The brain-specific S100B transgenic mice can be used in research on the role of S100B gene in the development of PD.

Key words: S100B, Parkinson's disease, dopamine receptor, G protein-coupled receptor kinase, dopamine

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