Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2017, Vol. 39 ›› Issue (3): 307-311.doi: 10.3881/j.issn.1000-503X.2017.03.002

• Orginal Article • Previous Articles     Next Articles

Hippocampal Apoptosis and Cognitive Function in Dysbindin-1B+/+ Mice

Wei YANG, Qi XU()   

  1. State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China
  • Received:2016-01-29 Online:2017-06-30 Published:2017-06-20

Abstract:

Objective To explore the apoptosis in the hippocampus of dysbindin-1B+/+ mice and the behaviors of 4-month-old dysbindin-1B+/+ mice and wild-type mice. Methods The hippocampus of dysbindin-1B+/+ mice and corresponding wild-type mice were assessed by TUNEL assay and transmission electron microscopy. Then twelve 4-month-old male dysbindin-1B+/+ mice and twelve wild-type mice were enrolled. The open field and T maze test were conducted to observe locomotor activity,exploratory behaviors,and spatial memory. Result TUNEL+ neurons were found in hippocampus of dysbindin-1B+/+ mice,especially in dentate gyrus region,but not in that of wild-type mice(t=12.98,P<0.0001). Transmission electron microscopy showed the presence of middle-and late-stage apoptosis in the hippocampus of dysbindin-1B+/+ mice,while there was no apoptosis in wild-type mice. Open field test showed that,compared with the moving distance of wild-type mice [(3632±606)cm],dysbindin-1B+/+ mice presented significantly lower moving distances [(2887±376)cm] (t=2.993,P=0.0122). Meanwhile,compared with the moving time of wild-type mice in the central area [(114.00±34.09)s],dysbindin-1B+/+ mice presented lower moving time [(64.22±14.67)s] (t=4.649,P=0.0023). T maze test showed that when the interval between forced run and test run were 60 s and 180 s,respectively,the accuracy of dysbindin-1B+/+ was (70.40±15.17)% and (59.28±9.10)%,which was significantly lower than those of wild type mice (91.67±13.94)% (t=3.261,P=0.0258) and (83.33±14.89)% (t=3.687,P=0.0291);when the interval was 15 s,there was no significant difference between these two groups [100% vs.(94.45±6.08)%; t=0.851,P=0.0756]. Conclusion Dysbindin-1B+/+ expression can cause the apoptosis of neurons,which may cause anxious behavior and impair the locomotor activity and spatial memory.

Key words: dysbindin-1, apoptosis, locomotor activity, exploratory behavior, cognition impairment

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