江苏地区汉族人群23个Y染色体STR基因座的多态性

doi:10.3881/j.issn.1000-503X.2017.05.006

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2017, Vol. 39 ›› Issue (5): 629-636.doi: 10.3881/j.issn.1000-503X.2017.05.006

• Orginal Article • Previous Articles Next Articles

Polymorphisms of 23 Y-chromosome STR Loci in A Han Population in Jiangsu Province

Jun MA¹, Aihua ZHU¹, Jinwei SHI¹, Ming JIN¹, Huajie BA¹, Zhiguang PING²()

¹Institute of Forensic Science of Public Security Bureau of Changzhou,Changzhou,Jiangsu 213003,China
²Department of Health Biostatistics,College of Public Health,Zhengzhou University,Zhengzhou 450001,China

Received:2016-07-14 Online:2017-10-30 Published:2017-10-30

Abstract

Abstract:

Objective To investigate the polymorphisms of 23 Y-STR loci in a Han population in Jiangsu province. Methods Blood samples were collected from 4821 unrelated healthy Han males in Jiangsu province. DNA templates were amplified by PowerPlex Y23 kit,and the amplification products were detected by 3500xL genetic analyzer. Then,we calculated the allele frequencies and gene diversities respectively,as well as the haplotype frequencies and haplotype diversities. Results The gene diversity of these 23 Y-STR loci ranged 0.4099-0.9696. A total of 4781 haplotypes were detected,of which 4743 were found once. The haplotype diversity was 0.99999812. Conclusion The 23 Y-STR loci used in this study are highly polymorphic in Han individuals in Jiangsu province and therefore suitable for population genetic study and forensic individual identification.

Key words: Y chromosome, short tandem repeat, genetic polymorphism

CLC Number:

Q347

Jun MA, Aihua ZHU, Jinwei SHI, Ming JIN, Huajie BA, Zhiguang PING. Polymorphisms of 23 Y-chromosome STR Loci in A Han Population in Jiangsu Province[J].Acta Academiae Medicinae Sinica, 2017, 39(5): 629-636.

Figures/Tables 4

Table 1

Allele frequencies and gene diversities of 22 Y-STR loci in a Han population in Jiangsu province(n=4281)"

等位基因 Allele	DYS19		DYS389Ⅰ			DYS389Ⅱ		DYS390	DYS391		DYS392		DYS393		DYS438		DYS439		DYS437		DYS448
6									0.0031
7									0.0002		0.0004				0.0006
8									0.0008		0.0002				0.0035		0.0006
9									0.0378				0.0002		0.0124		0.0012
10									0.7413		0.0097		0.0002		0.7812		0.0479
11			0.0118						0.2099		0.1037		0.0041		0.1738		0.4348
12	0.0027		0.5907						0.0068		0.1682		0.5038		0.0263		0.3798
13	0.0417		0.2392								0.3323		0.3130		0.0019		0.1166		0.0131
14	0.1888		0.1527								0.3379		0.1259		0.0002		0.0180		0.6227
15	0.4931		0.0054								0.0440		0.0508				0.0010		0.3427		0.0006
16	0.1929		0.0002								0.0033		0.0019						0.0216		0.0023
17	0.0792							0.0002			0.0002										0.0145
18	0.0017																				0.2580
19																					0.3595
20								0.0004													0.2512
21								0.0046													0.1016
22								0.0502													0.0110
23								0.4901													0.0012
24						0.0012		0.2732
25						0.0010		0.1639
26						0.0091		0.0156
27						0.1010		0.0017
28						0.3201		0.0002
29						0.2929
30						0.1971
31						0.0651
32						0.0106
33						0.0019
GD	0.7887		0.6844			0.8426		0.7283	0.4724		0.8159		0.7197		0.4099		0.7435		0.6589		0.8217
等位基因 Allele		DYS456		DYS458	DYS635		DY_GATA_H4			DYS481		DYS533		DYS549		DYS570		DYS576		DYS643
6
7																				0.0002
8												0.0002								0.0276
9							0.0008					0.0010		0.0006						0.0844
10							0.0434					0.0560		0.0029						0.2294
11							0.3107					0.6169		0.0699		0.0004				0.5175
12		0.0004		0.0006			0.5623					0.2728		0.5482						0.1230
13		0.0210		0.0054			0.0780					0.0400		0.3101		0.0004		0.0004		0.0172
14		0.1813		0.0201			0.0048					0.0129		0.0599		0.0139		0.0120		0.0006
15		0.4841		0.1908								0.0002		0.0079		0.0299		0.0120
16		0.1815		0.1811										0.0004		0.1528		0.0765
17		0.1064		0.2292	0.0006											0.2168		0.2132
18		0.0224		0.2072	0.0019					0.0004						0.2753		0.3093
19		0.0027		0.1056	0.1684					0.0029						0.1906		0.2381
20		0.0002		0.0446	0.2419					0.0075						0.0832		0.1064
21				0.0124	0.3203					0.0550						0.0268		0.0270
22				0.0027	0.1477					0.1576						0.0089		0.0035
23				0.0002	0.0809					0.2437						0.0008		0.0010
24					0.0322					0.2576						0.0002		0.0004
25					0.0052					0.1726
26					0.0010					0.0701
27										0.0189
28										0.0095
29										0.0025
30										0.0012
31										0.0004
32
33
GD		0.7735		0.8963	0.8679		0.6951			0.8735		0.6173		0.6797		0.8761		0.8552		0.7500

Table 1

Table 2

Table 3

Table 4

References 21

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位点 Locus	基因型 Genotypes	基因频率 Frequency	基因型 Genotypes	基因频率 Frequency	基因型 Genotypes	基因频率 Frequency	基因型 Genotypes	基因频率 Frequency
DYS385a/b	9/11	0.0002	11/19	0.0226	13/20	0.0282	15/22	0.0044
	9/12	0.0006	11/20	0.0089	13/21	0.0158	15/23	0.0023
	9/15	0.0002	11/21	0.0023	13/22	0.0050	15/24	0.0002
	9/18	0.0002	11/22	0.0004	13/23	0.0015	16	0.0029
	10	0.0006	12	0.0342	13/24	0.0010	16/17	0.0008
	10/11	0.0012	12/13	0.0392	13/25	0.0010	16/18	0.0008
	10/12	0.0019	12/14	0.0079	13/26	0.0002	16/19	0.0017
	10/13	0.0012	12/15	0.0085	14	0.0058	16/20	0.0017
	10/14	0.0004	12/16	0.0591	14/15	0.0025	16/21	0.0010
	10/16	0.0008	12/17	0.0587	14/16	0.0029	16/22	0.0008
	10/17	0.0019	12/18	0.0467	14/17	0.0137	16/23	0.0004
	10/18	0.0027	12/19	0.0510	14/18	0.0284	17	0.0002
	10/19	0.0021	12/20	0.0280	14/19	0.0162	17/18	0.0006
	10/20	0.0012	12/21	0.0089	14/20	0.0056	17/19	0.0002
	10/21	0.0002	12/22	0.0027	14/21	0.0023	17/20	0.0006
	10/22	0.0006	12/23	0.0023	14/22	0.0019	17/21	0.0008
	11	0.0141	12/24	0.0002	14/23	0.0006	18	0.0010
	11/12	0.0378	13	0.1104	14/26	0.0002	18/19	0.0004
	11/13	0.0114	13/14	0.0303	15/16	0.0056	18/20	0.0004
	11/14	0.0046	13/15	0.0048	15/17	0.0058	19	0.0002
	11/15	0.0029	13/16	0.0122	15/18	0.0048	19/20	0.0010
	11/16	0.0133	13/17	0.0266	15/19	0.0048	19/21	0.0002
	11/17	0.0214	13/18	0.0398	15/20	0.0050	20	0.0004
	11/18	0.0315	13/19	0.0535	15/21	0.0056	21	0.0002
GD	0.9696

单倍型Haplotype	MP	DC	HD
MHT	0.00142	0.5789	0.99894113
EHT	0.00085	0.6963	0.99944483
PPY12	0.00080	0.7239	0.99948822
YFiler	0.00028	0.9166	0.99994614
PPY23	0.00021	0.9917	0.99999812

基因座 Locus	江苏汉族Jiangsu Han	苏北汉族North of Jiangsu Han	河南汉族Henan Han	陕西汉族Shaanxi Han	内蒙汉族Inner Mongolia Han
DYS19	0.7887	0.8371	0.7115	0.7131	0.7361
DYS389Ⅰ	0.6844	0.7113	0.6087	0.6389	0.6608
DYS389Ⅱ	0.8426	0.8797	0.7653	0.7511	0.7590
DYS390	0.7283	0.7911	0.6806	0.7059	0.7227
DYS391	0.4724	0.4593	0.4127	0.4130	0.4017
DYS392	0.8159	0.8686	0.7433	0.7743	0.7826
DYS393	0.7197	0.7110	0.6309	0.6178	0.6421
DYS438	0.4099	0.4117	0.4971	0.5063	0.5569
DYS439	0.7435	0.8260	0.6886	0.6830	0.6974
DYS437	0.6589	0.6809	0.4730	0.5157	0.4838
DYS448	0.8217	0.8713	0.7288	0.7053	0.7267
DYS456	0.7735	0.7634	0.6671	0.6097	0.6531
DYS458	0.8963	0.9089	0.8018	0.8132	0.8907
DYS635	0.8679	0.8675	0.7980	0.7651	0.7716
DY_GATA_H4	0.6951	0.6763	0.6164	0.6282	0.6559
DYS385a/b	0.9696	0.9762	0.9647	0.9734	0.9345
基因座 Locus	湖北汉族Hubei Han	云南汉族Yunnan Han	广东汉族Guangdong Han	新疆维族Xinjiang Han	蒙古族Mongolian
DYS19	0.6653	0.6570	0.6858	0.7407	0.7153
DYS389Ⅰ	0.5941	0.6490	0.6130	0.5997	0.6590
DYS389Ⅱ	0.7316	0.7600	0.7658	0.7655	0.7271
DYS390	0.6937	0.6680	0.7229	0.7445	0.6772
DYS391	0.4057	0.4550	0.4285	0.4786	0.4326
DYS392	0.7257	0.7490	0.7143	0.6028	0.7858
DYS393	0.6408	0.6850	0.6680	0.6021	0.6520
DYS438	0.3838	0.3990	0.4855	0.6819	0.5095
DYS439	0.6498	0.7320	0.6633	0.7334	0.7215
DYS437	0.4623	0.3900	0.4829	0.5697	0.4370
DYS448	0.6965	0.7280	0.7392	0.6776	0.7223
DYS456	0.6577	0.6030	0.6013	0.6003	0.6855
DYS458	0.8114	0.7960	0.8169	0.8179	0.7910
DYS635	0.7758	0.7890	0.7852	0.7772	0.7809
DY_GATA_H4	0.6244	0.6430	0.6129	0.6859	0.6523
DYS385a/b	0.9650	0.9630	0.9654	0.9404	0.9296

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Polymorphisms of 23 Y-chromosome STR Loci in A Han Population in Jiangsu Province

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