HOXA5基因转录起始点上游区域高甲基化水平与神经管缺陷的关系

doi:10.3881/j.issn.1000-503X.2017.06.009

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2017, Vol. 39 ›› Issue (6): 785-791.doi: 10.3881/j.issn.1000-503X.2017.06.009

• Original Articles • Previous Articles Next Articles

Relationship of Methylation within Upper Stream Region of Transcription Starts Site

Ji ZHANG, Tian TIAN, Deqing YI, Linlin WANG(), Aiguo REN

Institute of Reproductive & Child Health,Ministry of Health Key Laboratory of Reproductive Health,Department of Epidemiology and Biostatistics,School of Public Health,Peking University,Beijing 100191,China

Received:2017-03-27 Online:2017-12-20 Published:2017-12-20
Supported by:
Supported by the National Natural Sciences Foundation of China (81472987)and the Beijing Natural Sciences Foundation (7162094)

Abstract

CLC Number:

R714.7

Ji ZHANG, Tian TIAN, Deqing YI, Linlin WANG, Aiguo REN. Relationship of Methylation within Upper Stream Region of Transcription Starts Site[J].Acta Academiae Medicinae Sinica, 2017, 39(6): 785-791.

Figures/Tables 4

Table 1

Table 2

Genome-wide methylation difference of TSS upper stream region within HOXA5 gene between case and control groups in the first stage"

分组Group	cg00969405	cg01323381	cg01370449	cg01748892	cg02005600	cg02106682
病例组Case group	0.43	0.30	0.26	0.22	0.30	0.41
对照组Control group	0.16	0.10	0.07	0.08	0.11	0.17
甲基化差异Difference of methylation level	0.27	0.20	0.19	0.14	0.19	0.24
t	5.36	5.61	4.48	3.90	4.60	4.72
P^a	0.002	0.002	0.013	0.016	0.009	0.003
分组Group	cg02646423	cg02916332	cg03207666	cg03368099	cg03744763	cg04863892
病例组Case group	0.31	0.26	0.54	0.22	0.32	0.20
对照组Control group	0.31	0.16	0.47	0.07	0.14	0.07
甲基化差异Difference of methylation level	0.00	0.09	0.07	0.15	0.18	0.13
t	0.02	3.54	1.80	5.30	2.71	3.57
P^a	0.989	0.041	0.214	0.002	0.075	0.048
分组Group	cg05774699	cg05835726	cg07049592	cg08070327	cg09880291	cg12015737
病例组Case group	0.41	0.28	0.48	0.53	0.55	0.41
对照组Control group	0.19	0.18	0.21	0.57	0.36	0.18
甲基化差异Difference of methylation level	0.22	0.09	0.27	-0.04	0.19	0.23
t	7.68	2.66	4.83	-0.53	5.94	4.92
P^a	<0.001	0.112	0.002	0.796	0.001	0.004
分组Group	cg12128839	cg13512268	cg13694927	cg14013695	cg14014955	cg14058329
病例组Case group	0.10	0.48	0.29	0.34	0.33	0.48
对照组Control group	0.03	0.26	0.21	0.25	0.12	0.37
甲基化差异Difference of methylation level	0.07	0.23	0.09	0.09	0.20	0.11
t	2.94	5.69	3.00	3.00	4.09	4.20
P^a	0.123	0.002	0.061	0.059	0.019	0.011
分组Group	cg14658493	cg14882265	cg16997642	cg17432857	cg17569124	cg19196335
病例组Case group	0.40	0.41	0.53	0.28	0.19	0.60
对照组Control group	0.26	0.36	0.36	0.21	0.07	0.49
甲基化差异Difference of methylation level	0.15	0.04	0.17	0.08	0.11	0.11
t	3.60	1.35	3.39	2.14	3.26	3.49
P^a	0.027	0.397	0.033	0.181	0.053	0.028
分组Group	cg19643053	cg19759481	cg20517050	cg20817131	cg23204968	cg23454797
病例组Case group	0.46	0.19	0.30	0.37	0.38	0.44
对照组Control group	0.22	0.06	0.17	0.23	0.15	0.33
甲基化差异Difference of methylation level	0.24	0.13	0.13	0.14	0.23	0.11
t	6.14	3.66	3.56	3.03	4.36	3.03
P^a	0.001	0.040	0.028	0.061	0.011	0.051
分组Group	cg24389585	cg25307665	cg25390165	cg25506432	cg26023912
病例组Case group	0.55	0.20	0.42	0.41	0.39
对照组Control group	0.39	0.08	0.23	0.58	0.30
甲基化差异Difference of methylation level	0.17	0.13	0.18	-0.17	0.10
t	5.09	3.95	6.68	-1.69	3.64
P^a	0.003	0.015	0.001	0.265	0.026

Table 2

Fig 1

Table 3

References 22

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分组Group	性别Sex		孕周Pregnant week
分组Group	男Male	女Female	<28周<28 weeks	≥28周≥28 weeks
对照组Control group	14(60.9)	9(39.1)	12(52.2)	11(47.8)
病例组Case group	21(40.4)	31(59.6)	36(69.2)	16(30.8)
合计Total number	35(46.7)	40(53.3)	48(64.0)	27(36.0)
χ²	2.689		2.014
P	0.101		0.156

分组Group	CpG_1.2	CpG_3	CpG_4	CpG_5	CpG_6.7
对照组Control group(n=23)	30.18± 9.62	18.91±10.23	18.52±10.99	29.57±10.10	19.30±11.53
所有NTDs组Total NTDs group(n=52)	40.15±16.38	30.98±15.45	31.00±15.84	38.44±14.09	31.06±16.97
脊柱裂组Spinal bifida group(n=29)	43.56±19.20	34.69±17.60	30.91±18.32	40.00±16.31	35.43±19.99
无脑畸形组Anencephaly group(n=23)	36.27±11.70	26.30±10.89	31.11±12.49	36.48±10.69	25.74±10.52
t¹	3.17	3.95	3.62	3.09	3.02
P¹	0.002	<0.001	0.001	0.003	0.004
t²	3.07	4.02	2.75	2.83	3.60
P²	0.004	<0.001	0.009	0.007	0.001
t³	1.89	2.35	3.35	2.25	1.98
P³	0.066	0.024	0.002	0.029	0.054
分组Group	CpG_8	CpG_9	CpG_10.11	CpG_12	CpG_13.14
对照组Control group(n=23)	19.65±10.82	9.54± 9.07	21.70±17.46	13.52± 8.58	22.83± 7.54
所有NTDs组Total NTDs group(n=52)	28.67±16.42	20.03±20.40	33.52±17.21	22.04±13.76	30.87±13.81
脊柱裂组Spinal bifida group(n=29)	32.38±18.45	25.71±23.83	36.83±18.52	25.19±13.33	31.24±17.17
无脑畸形组Anencephaly group(n=23)	23.77±12.00	12.62±11.96	29.35±14.75	18.14±13.59	30.39± 8.17
t¹	2.40	2.34	2.73	3.10	3.24
P¹	0.019	0.025	0.008	0.003	0.002
t²	3.10	2.56	3.00	3.47	2.37
P²	0.003	0.018	0.004	0.001	0.023
t³	1.21	0.74	1.61	1.32	3.26
P³	0.232	0.468	0.116	0.195	0.002

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