Acta Academiae Medicinae Sinica
Acta Academiae Medicinae Sinica ›› 2018, Vol. 40 ›› Issue (2): 178-186.doi: 10.3881/j.issn.1000-503X.2018.02.007
• Original Articles • Previous Articles Next Articles
LU Shihong, GE Meili, ZHENG Yizhou, YANG Shaoguang, CHEN Fang, YOU Yahong, HAN Zhongchao(
)
Received:2017-10-27
Online:2018-04-28
Published:2018-05-03
Supported by:CLC Number:
LU Shihong, GE Meili, ZHENG Yizhou, YANG Shaoguang, CHEN Fang, YOU Yahong, HAN Zhongchao. Effect of CD106+ Mesenchymal Stem Cell on Bone Marrow Vascular Failure in Patients with Aplastic Anemia[J].Acta Academiae Medicinae Sinica, 2018, 40(2): 178-186.
Fig 1
Identification of MSCs by fluorescence-activated cell sorting and immunohistochemical staining technique MSCs:mesenchymal stem cells;DAPI:4’,6-diamidino-2-phenylindole A.evaluating MSCs by fluorescence-activated cell sorting;B.identification of surface markers including CD13,CD29,CD73,CD166,CD34,and CD45 by immunofluorescence labeling [a1-a6:F-actin(green);b1-b6:the surface markers including CD13,CD29,CD73,CD166,CD34,and CD45(red),respectively;c1-c6:DAPI(blue);d1-d6:the corresponding merge]"
Fig 2
Comparison of the gene expression of AA MSCs and N MSCs AA:aplastic anemia;N:normalA.a clustering dendrogram was constructed according to the gene sequencing results of AA MSCs and N MSCs(the red indicates the up-regulated genes,and the green shows the down-regulated ones);B.the real-time polymerase chain reaction was used for the verification of vascular cell adhesion molecule-1(CD106) gene expression in AA MSCs and N MSCs"
Fig 3
Contents of CD106+MSCs in AA MSCs and N MSCs A.the levels of CD106+MSCs in N MSCs and AA MSCs were measured by fluorescence-activated cell sorting;B.the levels of CD106+MSCs in N MSCs and AA MSCs was compared"
Fig 4
The contents of CD106+MSCs in fresh bone marrow of AA MSCs and N MSCs A.the content of CD106+MSCs in N and AA fresh bone marrow MSCs was measured by fluorescence-activated cell sorting;B.the contents of CD106+MSCs in fresh bone marrow of N MSCs and AA MSCs were compared"
Fig 5
The MSCs from AA MSCs and N MSCs were induced into CD31+endothelial cells in vitro A.CD31+endothelial cells were detected by confocal microscopy;B.the proportion of CD31+cells induced from N MSCs and AA MSCs"
Fig 6
The CD31+cell differentiation from N MSCs and vascular cell adhesion molecule-1(CD106)-blocking N MSCs A.detection of CD31+endothelial cells differentiation from N MSCs and CD106-blocking N MSCs by confocal microscopy;B.the proportion of CD31+cells induced in N MSCs group and N block group"
Fig 7
The tubular structures formation in N MSCs group and N block group A.the tubular structures formation from N MSCs group and N block group by photomicrography;B.the total tube length of tubular structures in N MSCs group and N block group"
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