Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2018, Vol. 40 ›› Issue (2): 219-224.doi: 10.3881/j.issn.1000-503X.2018.02.012

• Original Articles • Previous Articles     Next Articles

Galectin3 Regulates Transforming Growth Factor-β-induced Epithelial-mesenchymal

Transition in A549 CellsWANG Yongqiang1, LIU Zihou1, SHU Jie1, TANG Shene2, LUO Fanyan1()   

  1. 1 Department of Cardiac Surgery, Central South University,Changsha 410008,China
    2 Institute of Medical Sciences,Xiangya Hospital,Central South University,Changsha 410008,China
  • Received:2017-06-14 Online:2018-04-28 Published:2018-05-03
  • Supported by:
    Supported by the National Natural Sciences Foundation of China (81572870) and the National Natural Sciences Foundation of Hunan (2016JJ4114)

Abstract:

Objective To explore the role of Galectin3 in transforming growth factor-β(TGF-β)-induced epithelial-mesenchymal transition (EMT) in A549 cells. Methods Galectin3 was over-expressed in an A549 cell line. EMT was induced in lung cancer A549 cells by adding TGF-β. The expressions of Galectin3,E-cadherin,and vimentin were determined by Western blot. The protein expression of E-cadherin and the morphological changes of the cells were detected by immunofluorescence. Cellular proliferation was analyzed with cell counting kit-8,and the cellular migration and invasion was measured by scratches healing and Transwell assay,respectively.Results When only Galectin3 was over-expressed in A549 cell line,the expression levels of EMT-related proteins such as E-cadherin and vimentin were not changed,and the abilities of cellular proliferation,invasion,and migration were not changed either. When the EMT was induced by TGF-β in A549 cells,the E-cadherin expression was down-regulated and the vimentin expression was up-regulated in A549 cells with Galectin3 over-expression. There was no significant change in cellular proliferation,whereas the abilities of cellular invasion and migration were enhanced.Conclusion The TGF-β-induced EMT in A549 cells can be enhanced by Galectin3.

Key words: Galectin3, epithelial-mesenchymal transition, transforming growth factor-β, lung cancer

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