Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2018, Vol. 40 ›› Issue (3): 360-364.doi: 10.3881/j.issn.1000-503X.2018.03.011

• Original Articles • Previous Articles     Next Articles

Application of Combined Special Staining in the Observation of Vascular Pathology

LI Yanwei,WANG Wei,MEI Shaoshuai,TENG Fei,CHEN Dong()   

  1. Department of Pathology,Beijing Anzhen Hospital,Capital University of Medical Science,Beijing 100029,China
  • Online:2018-06-30 Published:2018-07-05
  • Supported by:
    Supported by the Scientific and Technological Development Fund of Beijing Anzhen Hospital,Capital University of Medical Science(F201601)

Abstract:

Objective To evaluate the value of combined special staining technique in observing pathological changes in blood vessels.Methods Totally 999 vascular specimen were harvested from patients with complete medical records,clear diagnosis,and age≥18 years in the Pathology Department of Beijing Anzhen Hospital from January 2014 to September 2017.All specimen were stained with HE,Verhoef-Van Gieson,AB/PAS,and Masson. Then,the result of HE staining was compared with that of the combined special staining (Verhoef-Van Gieson,AB/PAS,and Masson).Results HE staining only showed a small amount of elastic fiber fracture. In contrast,the combined special staining clearly showed the pathological changes including fractures,decreased elastic fibers,and aggregation of extracellular mucous matrix. Diseases in these 999 patients included aneurysm of sinus of valsalva (SVA) (n=3),aortic root aneurysm (ARA) (n=177),thoracic aortic aneurysm (TAA) (n=78),abdominal aortic aneurysm (AAA) (n=17),total thoracoabdominal aortic aneurysm (tTAAA) (n=32),and aortic dissection (AD) (n=692). The median scores of combined special staining of SVA (Z=3.857,P=0.040),ARA (Z=14.307,P=0.000),TAA (Z=26.939,P=0.000),AAA (Z=22.412,P=0.000),tTAAA (Z=15.926,P=0.000),and AD (Z=39.213,P=0.000) were significantly higher than that of HE staining.Conclusion The combined Verhoeff-Van Gieson,AB/PAS,and Masson special staining is an effective technique for observing pathological changes of elastic fibers and mucus in blood vessels.

Key words: special stain, Verhoeff-Van Gieson stain, AB/PAS stain, Masson stain

CLC Number: