Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2006, Vol. 28 ›› Issue (6): 761-765.

• Original Articles • Previous Articles     Next Articles

Effect of Peroxisome Proliferator-Activated Receptor-α Agonist on Adipokines Expression in Rats Fed with High-fat Diet

LI Yan, HUANG Bin, CHENG Hua, LIANG Zhen, LIU Shan-ying   

  1. Department of Endocrinology, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China
  • Received:2006-07-04 Revised:1900-01-01 Online:2006-12-30 Published:2006-12-30
  • Contact: LI Yan

Abstract: ABSTRACT:Objective To explore the effect of peroxisome proliferator-activated receptor-α (PPAR-α) agonist fenofibrate on adipokines expression in high-fat diet fed SD rats and its relationship to insulin resis- tance (IR). Methods Rats were randomized into three groups (n=10):HD group, fed with high-fat diet; HDF group, fed with high fat diet and treated with fenofibrate; and control group, fed with normal diet. Animals were sacrificed after 4-week follow-up. Plasma lipids, fasting plasma insulin, free fatty acids (FFA), and insulin sensitivity were detected. Reverse transcription-polymerase chain reaction was used to semi-quantitatively determine the mRNA expression of adipokines including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), angiotensinogen (AGT), angiotensin Ⅱ type 1 receptor (AT1R), and adiponectin in brown fat. Results The plasma level of FFA, TG, and homeostatic model approach-IR index were(2.37±0.60)vs (1.59±0.30)vs(1.33±0.34 )mmol/L, (0.48±0.11)vs(0.30±0.04)vs(0.36±0.07)mmol/L, and 12.30±3.97 vs 5.03±1.88 vs 4.17±1.27 in the HD group, HDF group, and controlgroup after 4 weeks of treatment with fenofibrate, respectively. The mRNA expressions of TNF-α and adiponectin were 1.726±1.408 vs 0.713±0.711 vs 0.593±0.382 and 0.660±0.192 vs 0.949±0.35 vs 0.936±0.130 in these three groups, which showed significant difference between HD group and HDF group(P<0.05), while no significant difference between HDF group and control group (P>0.05). The mRNA expressions of AGT, AT1R, and IL-6 had no significant difference among these three groups(P>0.05). Conclusion PPAR-α agonist fenofibrate may reverse high-fat diet induced lipid abnormalities, improve insulin sensitivity, and regulate the mRNA expressions of TNF-α and adiponectin in adipose tissues.

Key words: peroxisome proliferator-activated receptor-α, aconist, adipokines, free fatty acid, insulin resistance