Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2006, Vol. 28 ›› Issue (6): 776-780.

• Original Articles • Previous Articles     Next Articles

Effect of Urotensin Ⅱ on Proliferative Potential and Phosphorylation of Extracellular Signal-regulated Kinase 1/2 of Adventitial Fibroblasts from Spontaneously Hypertensive Rat

DAI Hong-yan, GE Zhi-ming, LI Yong-hong   

  1. Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Shandong University, Jinan 250012, China
  • Received:2006-03-23 Revised:1900-01-01 Online:2006-12-30 Published:2006-12-30
  • Contact: GE Zhi-ming

Abstract: ABSTRACT:Objective To study the effect of urotensin Ⅱ(UⅡ) on the proliferative potential of adventitial fibroblasts(AFs) from spontaneously hypertensive rat(SHR) and to determine whether extracellular signal-regulated kinase 1/2 (ERK1/2) pathway is involved in this progress. Methods 3H-thymidine incorporation test was used to estimate the UⅡ-induced proliferative potential of AFs from SHR and the influence of Urantide(UⅡ receptor antagonist) and PD98059(ERK1/2 inhibitor). Western blotting was used to test the UⅡ-induced ERK1/2 phosphorylation as well as the effect of Urantide and PD98059 on UⅡ-induced ERK1/2 phosphorylation. Results UⅡincreased the proliferative potential of AFs from SHR in a dose-dependent way. Urantide and PD98059 wholly or partly inhibited UⅡ-induced proliferation of SHR-AFs. In SHR-AFs, UⅡinduced the phosphorylation of ERK1/2 in a time-dependent way, which was completely inhibited by Urantide and PD98059. Conclusion UⅡ can increase the proliferative potential of AFs from SHR and ERK1/2 pathway is partly involved in this progress.

Key words: urotensin Ⅱ, fibroblasts, proliferation, extracellular sicnal-reculated kinase 1/2