Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2007, Vol. 29 ›› Issue (6): 787-791.

• Original Articles • Previous Articles     Next Articles

Effects of 11,12-Epoxyeicosatrienoic Acid Preconditioning and Postconditioning on Ca2+- handling Proteins in Myocardial Ischemia/Reperfusion Injury in Rats

WANG Yan-xia,ZENG Xiang-jun ,LU Ling-qiao, MA Li-quan,JIANG Dong-qiao, MU Jing,WANG Xiao-yan, ZHANG Li-ke,TANG Chao-shu ,HAO Gang   

  1. Department of Pathophysiology, Capital Medical University, Beijing 100069, China
  • Received:2007-09-25 Revised:1900-01-01 Online:2007-12-30 Published:2007-12-30
  • Contact: HAO Gang

Abstract: ABSTRACT:Objective To investigate the effects of 11,12-epoxyeicosatrienoic acid(11,12-EET)preconditioning and postconditioning on Ca2+- handling proteins in myocardial ischemia/reperfusion(IR) injury in rats and reveal the effects and mechanism of 11,12-EET on cardioprotection. Methods The IR injury model was built by stopping perfusion for 40 minutes followed by reperfusion for 30 minutes. The isolated Langendorff-perfused rat hearts were divided into 4 groups: control group, IR group, EET preconditioning (Pre-EET) group and EET postconditioning (Post-EET) group. The computer-based electrophysiological recorder system was used to measure the changes of the maximal rate of pressure increased in the contraction phase (+dp/dtmax), the maximal rate of pressure decreased in the diastole phase (-dp/dtmax), the left ventricular end diastolic pressure (LVEDP) and the difference of left ventricular pressure (△LVP). The activity of Ca2+-ATPase in sarcoplasmic reticulum was measured with colorimetric method. Reverse transcription-polymerase chain reaction was used to assess the gene expression of Ca2+-handling protein [sarcoplasic reticulum Ca2+-ATPase(SERCA), phospholamban(PLB), ryanodine receptor type 2 (RyR2), and 1,4,5-trisphosphate inositol receptor type 2 (IP3R2)] mRNAs level. Results Compared with IR group, the myocardial functions, the value of Ca2+-ATPase, and the expressions of IP3R2 mRNA were significantly increased and the expression of PLB mRNA was significantly decreased in both Pre-EET group and Post-EET group (P<0.05,P<0.01). And the expression of SERCA mRNA was significantly increased in Pre-EET group (P<0.05). However, no significant differences were detected between Pre-EET and Post-EET groups. Moreover, the expression of RyR2 mRNA was not significantly different among all groups. Conclusions 11,12-EET preconditioning and postconditioning can protect myocardium from IR injury by elevating the activity of Ca2+-ATPase in sarcoplasmic reticulum, up-regulating the expression of IP3R2 mRNA, and down-regulating the expression of PLB mRNA. Moreover, up-regulating the expression of SERCA mRNA maybe one of mechanisms of 11,12-EET preconditioning on cardio protection against IR injury.

Key words: 11,12-epoxyeicosatrienoic acid, iscdemia/reperfusion injury, ca2+-dandlinc protein, preconditioninc, postconditioninc