Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2008, Vol. 30 ›› Issue (3): 280-283.

• Original Articles • Previous Articles     Next Articles

Effect of NECL1 on the Proliferation of T98G Glioma Cell Line

GAO Jing, CHEN Tao, YIN Bin, GONG Yan-hua, QIANG Bo-qin, YUAN Jian-gang, PENG Xiao-zhong   

  1. National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China
  • Received:2007-10-26 Revised:1900-01-01 Online:2008-06-30 Published:2008-06-30
  • Contact: GONG Yan-hua; PENG Xiao-zhong

Abstract: ABSTRACT:Objective To study the regulation role of tumor suppressor NECL1 on the proliferation of glioma cell line. Methods We detected the expression level of NECL1 in human normal brain tissue and glioma cell lines using semi-quantitative reverse transcription polymerase chain reaction(RT-PCR) and Western blot. T98G cell line in which NECL1 was silent and whose transfection efficiency was relatively high as target cell was chosen, and the effect of NECL1 on the proliferation of T98G cell line in vitro was detected by using cell growth curve, flow cytometry, and Hoechst staining. Results NECL1 was abrogated or markedly reduced in 6 glioma cell lines. When NECL1 was overexpressed in T98G cell line, the cell growth rate obviously decreased and the number of apoptotic cells remarkably increased when compared with the control group. Conclusion NECL1 may inhibit the proliferation of T98G cells by inducing its apoptosis.

Key words: necl1, t98c cell line, clioma, proliferation, apoptosis