Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2019, Vol. 41 ›› Issue (1): 28-36.doi: 10.3881/j.issn.1000-503X.10840

• Original Articles • Previous Articles     Next Articles

Effect of Aging on Experimental Colitis in Mice and Its Associated Mechanism

LIU Ailing1,WANG Hongying2,Lü Hong1(),QIAN Jiaming1   

  1. 1 Department of Gastroenterology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China
    2 Key Laboratory of Molecular Oncology,Cancer Hospital,CAMS and PUMC,Beijing 100021,China
  • Received:2018-09-11 Online:2019-02-28 Published:2019-03-06
  • Contact: Hong Lü
  • Supported by:
    Supported by the Health Research & Special Projects Grant of China(201002020);the Chinese Academy of Medical Sciences Medicine and Health Technology Innovation Project(2016-I2M-3-001);the National Natural Sciences Foundation of China(81570505);2010 National Clinical Key Specialist Project and the National Science and Technology Ministry 973 Plan(2015CB943203)


Objective To explore whether aging increases severity of colitis in mice and its mechanism.Methods Young (6-8 weeks)and aged (56 weeks) C57Bl/6 mice were divided into the control and experimental group (n=5,each). Dextran sodium sulfate(DSS) was used to induce acute colitis mouse model in the experimental group.The mRNA expressions of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in colon were measured by RT-PCR. Tight junctions (TJs) of intestinal epithelial cells was examined by transmission electron microscopy (TEM). Protein expressions of E-cadherin and occludin were detected by Western blotting and immunohistochemistry in colon.Results Compared with the young DSS-induced mice,the aged DSS-induced mice had more weight loss(t=3.679,P=0.006),higher disease indexes (t=2.496,P=0.037),higher histologic scores(U=0.000,P=0.008) and higher colonic IL-6 level (U=4.000,P=0.191). The TJs of intestinal epithelial cells were discontinuous in old healthy rats,and the TJs were destroyed significantly in both young and aged DSS-induced mice. Compared with the young DSS-induced mice,the aged DSS-induced mice had decreased protein expressions of E-cadherin (t=0.184,P=0.863)and occludin (t=0.399,P=0.710).Conclusions Aging leads to more severe disease following DSS challenge. Age-related deterioration in the functions of the gastrointestinal barrier and integrity may be one of the possible mechanisms.

Key words: aging, inflammatory bowel disease, tight junction, intestinal barrier

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