Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2019, Vol. 41 ›› Issue (1): 37-42.doi: 10.3881/j.issn.1000-503X.10456

• Original Articles • Previous Articles     Next Articles

Mechanism of Th1/Th2 Cytokines in HLA-DQ8 Transgenic Mice Model of Ocular Experimental Autoimmune Myasthenia Gravis

WU Xiaorong1(),XIONG Yingqiong2,HU Yuxiang1,XU Xiaoxuan1,CHENG Yi1,YAN Li1,WU Yajun1,RAO Jie1   

  1. 1 Department of Ophthalmology,the First Affiliated Hospital of Nanchang University,Nanchang 330006,China
    2 Department of Neurology,Jiangxi Provincial People’s Hospital,Nanchang 330006,China;
  • Received:2018-04-13 Online:2019-02-28 Published:2019-03-06
  • Contact: Xiaorong WU E-mail:wxr98021@126.com
  • Supported by:
    Supported by the National Natural Sciences Foundation of China(81760179);Supported by the National Natural Sciences Foundation of China(81360151);the Natural Science Foundation of Jiangxi Province(20171BAB205046);the Key Foundation of Education Department of Jiangxi Province(GJJ160033);the Health Development Planning Commission Science Foundation of Jiangxi Province(20185118)

Abstract:

Objective To analyze the levels of cytokines (IL-2,IFN-γ,IL-6,IL-10) associated with Th1 and Th2 cells in HLA-DQ8 transgenic mice model of ocular experimental autoimmune myasthenia gravis (oEAMG) induced by recombinant H-AChR γ subunit immunization.Methods DQ8 mice were immunized with 20 μg of AChR γ subunit,20 μg of crude E. coli extract (E. coli group),or complete Freund’s adjuvant (CFA) only (CFA group). All mice were immunized on days 0,30,and 60. Mice were euthanized 28 days after the third immunization,and draining lymph node cells (LNC) and spleen lymphocytes were cultured in vitro. The supernatant was collected to observe the interleukin(IL)-2,interferon(IFN)-γ,IL-6,IL-10 production by ELISA.Results LNCs and spleen lymphocytes of H-AChR γ subunit-immunized mice exhibited significantly enhanced IFN-γ (F=76.332,P<0.001;F=34.865,P<0.001) and IL-2 (F=42.835,P<0.001;F=38.030,P<0.001),which associated with Th1 cells,as compared to E. coli group and CFA group. There were no significant differences in IL-6 (F=1.325,P=0.284;F=1.935,P=0.166) and IL-10 (F=0.908,P=0.417;F=1.189,P=0.322) levels,which secreted by Th2 cells,among these three groups.Conclusion Th1 cytokines play key roles in the pathogenesis of oEAMG,while the mechanism of Th2 cytokines for oEAMG remains unclear.

Key words: ocular experimental autoimmune myasthenia gravis, acetylcholine receptor γ subunit;, Th1/Th2, cytokine

CLC Number: