Acta Academiae Medicinae Sinica

Abstract: ABSTRACT:Objective To investigate the relationship between the resuscitation promoting role of resuscitation promoting factor and the initial bacteria amount of dormant Mycobacterium tuberculosis. Methods Mycobacterium tuberculosis (dormant bacteria) was cultured for 100 days, then diluted into 1 mg/ml concentration with 7H9, and further diluted into 0.5, 0.25, 0.125, 0.0625, and 0.03125 mg/ml. Twelve new tubes added with 5 ml 7H9 and divided into two groups: the first group was added with the resuscitation-promoting factor protein, and the second group as control was added with 7H9. In each group the above diluted solutions were added. The tubes were located at 37℃ for culture. Optical density (OD) was detected on day 15, 25, 30, and 35. From each tube 1 μl culture solution was plated on 7H11 medium for colony counting. Results OD detection showed that bacteria proliferation in each group had positive linear correlation (P<0.05, P<0.01), indicating that the resuscitation-promoting factor played a similiar role in solutions with different dilution concentrations. 7H11 results and the OD results show that these two detection methods in each group had linear correlation (P<0.05, P<0.01), indicating that these two methods showed consistent test results. Conclusion The resuscitation-promoting factor has no effect on the resuscitation of dormant Mycobacterium tuberculosis and its initial bacteria amount.

Key words: mycobacterium tuberculosis, resuscitation promotinc factor, culture