Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2009, Vol. 31 ›› Issue (5): 547-550.doi: 10.3881/j.issn.1000-503X.2009.05.007

• Original Articles • Previous Articles     Next Articles

Application of Fluorescence in situ Hybridization and Flow Cytometry in the Measurement of Telemere Length in Chinese

LIU Bo1;CUI Wei2;HAN Bing1; WANG Xuan1; LIN Jie3;ZHAO Yong-qiang1   

  1. 1Department of Hematology,2Department of Clinical Laboratory, PUMC Hospital, CAMS and PUMC, Beijing 100730, China

    3Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China

  • Received:2009-03-23 Revised:1900-01-01 Online:2009-10-30 Published:2009-10-30
  • Contact: HAN Bing

Abstract: ABSTRACT:Objective To measure the telomere length in patients with bone marrow failure syndrome (BMF) using fluorescence in situ hybridization and flow cytometry (Flow-FISH). Methods Blood samples were collected from 8 patients with BMF. Telomere lengths of mononucleared cells from 8 BMF patients and granulocytes from 3 of these 8 BMF patients (all these 3 patients were suffered from aplastic anemia) were measured using Flow-FISH, and the results were compared with those of normal controls. Telomere lengths were measured by Southern blot in 3 patients, and the results were compared with those obtained from Flow-FISH. Results Among these 8 BMF patients, 5 have decreased telomere length in their mononucleared cells. However, different white cell subgroups, specifically mononucleated cells and granulocytes, have different degree of telomere shortening. In 3 BMF patients who underwent both Flow-FISH and Southern blot, 2 patients had consistent measurement results. Conclusion Flow-FISH can be used for the measurement of telomere length in Chinese patients with BMF.

Key words: bone marrow failure syndrome, telomere lenctd, fluorescence in situ dybridization and flow cytometry