Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2012, Vol. 34 ›› Issue (2): 109-115.doi: 10.3881/j.issn.1000-503X.2012.02.003

• Original Articles • Previous Articles     Next Articles

Creation of An Animal Model for Post-operative Adhesion Prevention

REN Chang1, ZHU Lan1, SUN Ji-chao2   

  1. 1Department of Obstetrics & Gynecology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China;2Centre of Drug Safety Evaluation and Research, Institute of Laboratory Animal Sciences,CAMS and PUMC, Beijing 100032,China
  • Received:2011-06-30 Revised:2012-04-28 Online:2012-04-28 Published:2012-04-28
  • Contact: ZHU Lan

Abstract: Objective To establish a rabbit double uterine horn model for assessing the time-course of pelvic adhesions and evaluating the effectiveness of different anti-adhesive materials in reducing adhesions. Methods A total of 330 Japanese white rabbits underwent laparotomy, followed by uterine horn incision. Animals were euthanized after 3, 7, 14, 28, and 42 days, respectively. Results The surgical procedure was smooth. Rabbit double uterine horn model was applied for the evaluation of pelvic adhesions in a three-dimension fashion. Each of the three means-gauze abrasion, needle holder clamping, and direct uterine incision induced postoperative pelvic adhesions, among which direct uterine incision was the best to mimic conditions after gynecological operations. Under normal circumstances, degradation of filmy fibrinous adhesions by locally released proteases of the fibrinolytic system occured within 3 days of injury. The regeneration of the mesothelium was completed within 7 days. Collagen reached its peak by day 14. Anti-adhesive materials were supposed to be completely absorbed by day 28, and their effectiveness in preventing pelvic adhesions was confirmed at this time point. Whether their effect can be maintained after the absorption of the anti-adhesive materials was investigated in 42 days. Conclusions The animal model was successfully established. It well mimics the postoperative pelvic adhesions after direct uterine horn injury and thus is a suitable model for studying site-specific adhesions. Observations on the 3rd, 7th, 14th, 28th, and 42nd post-operative days provided a full picture of the adhesion formation process.

Key words: uterus, rabbit, animal model, surgery, pelvic adhesion, anti-adhesive barrier