Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2012, Vol. 34 ›› Issue (3): 197-201.doi: 10.3881/j.issn.1000-503X.2012.03.001

• Original Articles •     Next Articles

Inhibitory Effect of A Ras Homologue Member I on Pancreatic Cancer andIts Modulation on Epithelium Growth Factor Receptor-Ras-Raf-Mitogen-Activated Protein Kinase/Extracellular Regulated Protein Kinase/1/2 Pathway

LU Jia1, QIAN Jia-ming1, YANG Hong1, LI Jing-nan1, XU Feng-ji2   

  1. 1Department of Gastroenterology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China
    2Department of Urinary Surgery, Wuxi Hemujia Hospital, Wuxi, Jiangsu 214101, China
  • Received:2012-02-15 Revised:2012-06-30 Online:2012-06-29 Published:2012-06-29
  • Supported by:

    Supported by the National Natural Sciences Foundation of China(39830350)

Abstract: Objective To investigate the regulation of epithelium growth factor receptor (EGFR), pan-Ras, and extracellular regulated protein kinase (ERK) with both a ras homologue member I (ARHI) suppression and epithelium growth factor (EGF) stimulation. Methods After identification and implication, the constructed plasmid pIRES2-EGFP-ARHI was transfected into Panc-1. The untransfected cell was also explored as controls. The growth curve was drawn to indicate the proliferation effect of ARHI. EGFR-ELISA was performed to investigate the expression of EGFR. Western blot analysis was used to investigate the expression of protein MAPK/ERK1/2, pan-Ras in Panc-1. Results The proliferation rate of Panc-1 was inhibited by ARHI compared with both empty plasmid and untransfected cell. The amount of EGFR was parallel in both transfected and untrasfected cell but affected by EGF stimulation. The amount of pan-Ras was decreased after ARHI transfection. The optimum concentration of EGF effect on P-ERK was 50 ng/ml. Conclusion Both ARHI and EGF play roles in the EGF-EGFR-Ras-Raf-MAPK/ERK1/2 pathway.

Key words: a ras homologue member I, epithelium growth factor, epithelium growth factor receptor, Ras, pancreatic cancer

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