Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2012, Vol. 34 ›› Issue (4): 348-352.doi: 10.3881/j.issn.1000-503X.2012.04.007

• Original Articles • Previous Articles     Next Articles

Effect of Adenovirus-mediated Basic Fibroblast Growth Factor Gene Transfer in vivo on Oligodendrocyte Cell Numbers Throughout Ventrolateral White Matter Following Spinal Cord Injury in Rats

YANG Yan-ling   

  1. Department of Physiology, Medical College of Yan'an University, Yan'an, Shaanxi 716000, China
  • Received:2012-04-11 Revised:2012-08-31 Online:2012-08-30 Published:2012-08-30
  • Supported by:

    Supported by the Scientific Research Program for the Education Department in Shaanxi Province (12JK0705)

Abstract: Objective To study the effect of adenovirus-mediated basic fibroblast growth factor(FGF-2) gene transfer in vivo on oligodendrocyte cell numbers throughout ventrolateral white matter following spinal cord injury in rats. Methods Thirty-two adult female Sprague Dawley rats were injured with the Infinite Horizon Impactor, and then were randomly assigned to four groups: FGF-2-Adts high-titer group (1.27x107 pfu/rat), FGF-2-Adts intermediate-titre group (6.37x106 pfu/rat), FGF-2-Adts low-titer group (3.18 x 106 pfu/rat), and green fluorescent protein (GFP)-Adts group (5.9x107 pfu/rat). The transgenic expression in vivo was detected with fluorescence microscopy. The locomotor function of the hindlimbs of rats was evaluated using Rivlin plate. Slides mounted with tissue sections were processed for immunohistochemical detection and quantification of oligodendrocytes (CC1+) in the ventral lateral funiculi (VLF) of injured spinal cords. Results One week after spinal cord injury, GFP showed that many cells had expressed objective gene in vivo and the angles of the occlusal plane of rats in FGF-2 groups were significantly higher than in GFP-Adts group. Also, there was a significant difference among the FGF-2-Adts treatment groups for the volume of gray matter sparing. However, there were no significant differences for total white matter sparing. Stereological quantification of total CC1+ cell numbers in the spared VLF showed a significant reduction in numbers with GFP controls compared to all other groups 4 weeks after injury. In contrast, the FGF-2 Adts intermediate-titer group had significantly more CC1+ cells when compared to both the FGF-2-Adts high- and low-titer groups. Conclusion Adenovirus-mediated FGF-2 gene transfer can promote the functional recovery of the injured spinal cord by enhancing the proliferation and/or differentiation of oligodendrocytes.

Key words: spinal cord injury, basic fibroblast growth factor, oligodendrocyte cell, adenoviruses vector, gene therapy

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