Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2012, Vol. 34 ›› Issue (4): 353-358.doi: 10.3881/j.issn.1000-503X.2012.04.008

• Original Articles • Previous Articles     Next Articles

Effect of 3.6-mT Sinusoidal Electromagnetic Fields on Proliferation and Differentiation of Osteoblasts In Vitro

ZHOU Jian1, WANG Jia-qi1, GE Bao-feng1, MA Xiao-ni1, CHEN Ke-ming1, WEI Zhe2   

  1. 1Institute of Orthopaedics,2Department of Medical Engineering, Lanzhou General Hospital of Lanzhou Military Area
    Command of Chinese PLA, Lanzhou 730050, China
  • Received:2011-07-19 Revised:2012-08-31 Online:2012-08-30 Published:2012-08-30
  • Supported by:

    Supported by the Grant from Gansu Provincial Science and Technology Department(09ZNKDA025)

Abstract: Objective To investigated the effect of 50-Hz 3.6-mT sinusoidal electromagnetic fields (SEMFs) on the proliferation and differentiation of osteoblasts in vitro. Methods The newborn rat calvarial osteoblasts were isolated by enzyme digestion and randomly divided into 6 groups after one passage. The treatment groups under 50-Hz 3.6-mT SEMFs and controls without SEMFs treatment. The cells were exposed in the SEMFs for 0.5 h, 1.0 h, 1.5 h, 2.0 h, and 2.5 h. They were observed under the contrast phase microscope each day. The calcified nodules were stained by alizarin red. The SEMFs were arranged in spiral appearance after 3 to 5 days. Results The SEMFs showed characteristic distribution 3 to 5 days after SEMFs treatment. On the 9th day after treatment, the activity of alkaline phosphatase (ALP) significantly increased in the 0.5-h group, whereas the ALP histochemical straining results and the area of calcified nodules were consistent with ALP activity. In the 48-h and 96-h groups, the genetic expression levels of osteoprotegerin and collagen-Ⅰ were significantly higher than that in the control group; particularly, the mRNA expression increased in the 0.5-h group. Conclusion The SEMFs at 50-Hz 3.6-mT could suppress the proliferation of osteoblasts maturation but stimulate the differentiation and maturation of osteoblasts in vitro.

Key words: osteoblasts, sinusoidal electromagnetic fields, collagen-Ⅰ, osteoprotegerin

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