Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2015, Vol. 37 ›› Issue (1): 12-16.doi: 10.3881/j.issn.1000-503X.2015.01.003

• Orginal Article • Previous Articles     Next Articles

Effects of External Magnetic Field on the Transfection Rate of SPIO-shRNA Dual Functional Molecular Probe into Ovarian Carcinoma SKOV3 Cells in Vitro

Xiao-dong GE, Mei-ling LI, Xiao-lin DENG, Xiao-feng WU, Dan-ni ZENG, Rui-kun LIAO, Ming WEN(), Shao-lin LI   

  1. Department of Radiology,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China
  • Received:2014-05-22 Online:2015-02-28 Published:2015-02-12
  • Supported by:
    Supported by the National Natural Sciences Foundation of China(81171366)and the National Clinical Key Subject Construction Project(2013-544)

Abstract:

Objective To explore the transfection rate of SPIO-shRNA dual functional molecular probe into ovarian carcinoma SKOV3 cells in external magnetic field. Methods Dual functional molecular probe at an iron concentration of 45 mg/L was transfected into SKOV3 cells. The cells with coexisting probe and magnetic fields were set as the intervention group,the probe-transfected cells as negative control group,and normally cultured SKOV3 without any transfection as blank control group. The transfection rate was detected by flow cytometry. Cell viability was observed by CCK-8 assay. Epidermal growth factor receptor(EGFR) expression level in SKOV3 cells was determined by real-time quantitative PCR and Western blot analysis.The signal intensity was measured by magnetic resonance imaging(MRI). Results The transfection rate of the intervention group was (79.20±3.31)%,which was significantly higher than that of negative control group(P=0.001). Compared with the negative control group,the cell viability of the intervention group significantly decreased(P=0.011), protein and mRNA expression levels of EGFR in the intervention group were significantly decreased(both P<0.05). The signal intensity on T2*WI in the intervenion group also significantly decreased(P=0.0004). Conclusion The external magnetic field can improve the transfection efficiency SPIO-shRNA dual functional molecular probe into ovarian carcinoma SKOV3 cells.

Key words: molecular probe, magnetic field, SKOV3 cells, transfection

CLC Number: