Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2016, Vol. 38 ›› Issue (4): 404-410.doi: 10.3881/j.issn.1000-503X.2016.04.007

• Orginal Article • Previous Articles     Next Articles

Identification of Epitopes for Neutralizing Antibodies Against H10N8 Avian Influenza Virus Hemagglutinin

Jin-fang HU1, Chun-yun SUN2, Mu-ding RAO3, Liang-zhi XIE1()   

  1. 1Cell Engineering Center,CAMS and PUMC,Beijing 100005,China
    2New Drug R&D Department,Sinocelltech Ltd,Beijing 100176,China;
    3Molecular Biology R&D Department,Sino Biological Inc,Beijing 100176,China;
  • Received:2016-03-04 Online:2016-08-20 Published:2016-08-20

Abstract:

Objective To develop neutralizing monoclonal antibodies (MAbs) against H10N8 avian influenza virus hemagglutinin and to identify the binding sites. Methods MAbs against hemagglutinin of H10N8 avian influenza virus were developed by genetic engineering. Neutralizing MAbs were screened by microneutralization assay,and then tested by enzyme-linked immunosorbent assay and Western blot to identity the binding sites.The homology modeling process was performed using Discovery Studio 3.5 software,while the binding epitopes were analyzed by BioEdit software. Results One MAb that could neutralize the H10N8 pseudovirus was obtained and characterized. Analysis about epitopes suggested that the antibody could bind to the HA1 region of hemagglutinin,while the epitopes on antigen were conserved in H10 subtypes.Conclusions One neutralizing antibody was obtained by this research.The MAb may potentially be further developed as a pre-clinical candidate to treat avian influenza H10N8 virus infection.

Key words: avian influenza H10N8 virus, hemagglutinin, neutralizing antibody, epitopes

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