Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2017, Vol. 39 ›› Issue (6): 737-742.doi: 10.3881/j.issn.1000-503X.2017.06.001

• Original Articles •     Next Articles

Correlation between the Expression of High Temperature Requirement Serine Protease A1 in Nucleus Pulposus Tissue and the Degree of Intervertebral Disc Degeneration

Dapeng LI1, Yan WU2, Jiawei YUE2, Jifu SUN1, Jialun WANG2, Yonghui HUANG1()   

  1. 1Department of Orthopaedics,Affiliated Hospital of Jiangsu University,Zhenjiang,Jiangsu 212001,China
    2Department of Physiology,Medical College of Jiangsu University,Zhenjiang,Jiangsu 212013,China
  • Received:2016-10-26 Online:2017-12-20 Published:2017-12-20
  • Supported by:
    Supported by the National Natural Sciences Foundation of China (81601931),Natural Sciences Foundation of Jiangsu Province (BK20150475),and Key Research and Development Programs of Zhenjiang City (SH2015085)

Abstract:

Objective To investigate the correlation between the expression level of high temperature requirement serine protease A1 (HtrA1) in nucleus pulposus and the degree of intervertebral disc degeneration (Pfirrmann grade).Methods Thirty-six patients who underwent excision of nucleus pulposus were examined by magnetic resonance imaging (MRI) before operation,and the Pfirrmann grading of all patients was performed according to the sagittal T2 weighted MRI.The expression of HtrA1 in nucleus pulposus tissue was detected by reverse transcription polymerase chain reaction and Western blot.The correlation between the expression level of HtrA1 in nucleus pulposus tissue and Pfirrmann grade was analyzed.Results MRI in all 36 patients showed that there were 3 cases of Pfirrmann grade Ⅰ,10 cases of grade Ⅱ,11 cases of grade Ⅲ,7 cases of grade Ⅳ,and 5 cases of grade Ⅴ.The mRNA and protein expressions of HtrA1 in nucleus pulposus tissue increased with the increase of Pfirrmann grade.There were significant differences in the expression level of HtrA1 among different Pfirrmann grade groups (P<0.05) except for the difference between grade Ⅳ and Ⅴ (P>0.05).Spearman rank correlation analysis revealed that there was a rank correlation between expression level of HtrA1 and Pfirrmann grade (P<0.000 1).Conclusion The mRNA and protein expressions of HtrA1 in nucleus pulposus tissue increase with the increase of Pfirrmann grade,indicating HtrA1 is correlated with the degree of intervertebral disc degeneration.

Key words: intervertebral disc degeneration, nucleus pulposus, magnetic resonance imaging, Pfirrmann grading

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