Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

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Effects of Gui Zhi Fu Ling Jiang Nang on Hemorheology, Expression of Phenotypic Proteins in Uterine Smooth Muscle Cells in Rats with An Intrauterine Device

LU Ying1, ZHU Yingjun2, YUE Xiuying1, CHEN Shaozheng1, WANG Xiong3   

  1. 1Department of Gynecology,Baodi Clinical College of Tianjin Medical University,Tianjin 301800, China;
    2Department of General Gynecology,Tianjin Central Hospital of Gynecology Obstetrics,Tianjin 300100, China;
    3Department of Ultrasonography, Baodi Clinical College of Tianjin Medical University Tianjin 301800, China
  • Received:2017-06-09 Online:2018-05-03 Published:2018-05-03
  • Contact: ZHU Yingjun Tel: 022-58287129,E-mail:zhuyj8072@sina.com

Abstract: Objective To observe the effect of Gui Zhi Fu Ling Jiang Nang (GZFLJN) on the expressions of alpha smooth muscle actin (α-SMA) and proliferating cell nuclear antigen (PCNA) in uterine vascular smooth muscle cells (VSMC) of rat models with an intrauterine device (IUD) and to determine the thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) levels in peripheral blood. Methods Female Wistar rats were randomly divided into four groups: normal group (n=16, with normal breed without treatment), model group (n=18, drenching 0.9% normal saline after modeling of IUD), GZFLJN group (n=18), and aminocaproic acid tablets group (n=17). Immunohistochemical SP (steptavidin -perosidase) method was used to detect the expressions of a-SMA and PCNA in uterine VAMC. Enzyme-linked immunosorbent assay (ELISA) was served to detect the levels of TXB2 and 6-keto-PGF1α in peripheral blood. Results The positive rate of a-SMA were (50.89±9.41)%, (26.93±6.80)%, (48.92±6.80)%, and (34.63±7.26)%, respectively, in normal group, model group, GZFLJN group, and aminocaproic acid tablets group; obviously, it was significantly higher in normal group (t=14.43,P=0.00) and GZFLJN group (t=11.37,P=0.00) than that in model group and it was significantly lower in aminocaproic acid tablets group than in normal group (t=9.96,P=0.00) and GZFLJN group (t=8.23,P=0.00). The positive rate of PCNA were (25.66±7.24)%, (61.26±9.98)%, (28.36±9.17)%, and (50.23±8.71)%, respectively, in these four groups; obviously, it was significantly lower in the normal group (t=20.86,P=0.00) and GZFLJN group (t=19.12,P=0.00) than in model group and it was significantly higher in aminocaproic acid tablets group than in normal group (t=17.82,P=0.00) and GZFLJN group (t=16.05,P=0.00). Serum TXB2 level in these four groups were (445.86±24.43)ng/L, (508.78±12.42)ng/L, (448.11±9.63)ng/L, and (498.11±13.63)ng/L; obviously, it was significantly higher in model group than in normal group (t=16.55,P=0.00) and aminocaproic acid tablets group (t=﹣4.12,P=0.00) and it was significantly lower in GZFLJN group than in model group (t=-15.23,P=0.00) and aminocaproic acid tablets group (t=-12.08, P=0.00). Serum 6-keto-PGF1α level in these four groups were (23.17±1.93)ng/L, (18.09±0.93)ng/L, (22.70±1.61)ng/L, and (20.70±1.41)ng/L, respectively; obviously, it was significantly lower in model group than in normal group (t=﹣13.98,P=0.00) and aminocaproic acid tablets group (t=5.26, P=0.00) and it was significantly higher in GZFLJN group than in model group (t=11.43,P=0.00) and aminocaproic acid tablets group (t=8.76,P=0.00). Conclusion GZFLJN can regulate the expressions of a-SMA and PCNA of VSMC in the endometrium of IUD rats and the concentrations of TXB2 and 6-keto-PGF1α in the serum.

Key words: intrauterine device model rats, Guizhifuling capsule, α-smooth muscle actin, proliferating cell nuclear antigen, thromboxane B2, 6-keto-prostaglandin F1α

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