Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2006, Vol. 28 ›› Issue (6): 766-769.

• Original Articles • Previous Articles     Next Articles

Expression of Renin-Angiotensin-Aldosterone System in
Human Adipose Tissues

ZHANG Xue-han, ZENG Zheng-pei, LI Han-zhong, ZHOU Ya-ru,
ZHANG Jing, TONG An-li, YAN Zhao-li

  1. Department of Endocrinology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China
  • Received:2006-08-28 Revised:1900-01-01 Online:2006-12-30 Published:2006-12-30
  • Contact: ZENG Zheng-pei

Abstract: ABSTRACT:Objective To compare the mRNA expression of renin-angiotensin-aldosterone system in human subcutaneous and visceral adipose tissues. Methods Total RNA was extracted from 12 human subcutaneous adipose tissues, 12 perirenal adipose tissues and 9 periadrenal adipose tissues. The expressions of angiotensinogen (AGT), renin, angiotensin converting enzyme(ACE), angiotensin converting enzyme 2 (ACE2), angiotensin Ⅱ receptor type 1 (AT1), angiotensin Ⅱ receptor type 2 (AT2), CYP11B2, and their internal reference glyceraldehyde phosphate (GAPDH) were studied by reverse transcription-polymerase chain reaction. The ratios of each target genes were used to evaluate the expression levels of AGT, renin, ACE, ACE2, AT1, AT2, and CYP11B2 in different adipose tissues. Results The mRNA expressions of AGT, ACE, ACE2, AT1, and AT2 were detected in human subcutaneous, perirenal, and periadrenal adipose tissues. However, CYP11B2 mRNA expression was not found in these three adipose ti- ssues. The mRNA expressions of renin was only detected in perirenal and periadrenal adipose tissues, which was significantly higher in perirenal adipose tissues than in periadrenal adipose tissues(P<0.05=. The mRNA expressions of ACE and ACE2 in perirenal adipose tissues were significantly higher than that in subcutaneous adipose tissues (P<0.05). The mRNA expressions of ACE were significantly higher than that of ACE2 in subcutaneous, perirenal, and periadrenal adipose tissues(P<0.05). The mRNA expressions of AT1 were significantly lower than that of AT2 in periadrenal adipose tissues (P<0.05). Conclusion Local renin-angiotensin system exists in the adipose tissues; however, aldosterone is not synthesized in the adipose tissues.

Key words: renin-anciotensin-aldosterone system, subcutaneous adipose tissue, perirenal adipose tissue, periadrenal adipose tissue