Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica

Acta Academiae Medicinae Sinica ›› 2006, Vol. 28 ›› Issue (6): 813-816.

• Original Articles • Previous Articles     Next Articles

Effects of Butyrylchitosan on Expression of Proliferating Cell Nuclear Antigen in ibroblast Proliferation of Rabbit Eyes after Filtering Operation

WEI Ping, WANG Ai-qin, Lei Xun-wen, CAO Zhi-xin, LI Xiao-lin, WANG Qin   

  1. Department of Ophthalmology, the First People’s Hospital of Lanzhou City, Lanzhou 730050, China
  • Received:2006-03-17 Revised:1900-01-01 Online:2006-12-30 Published:2006-12-30
  • Contact: WEI Ping

Abstract: ABSTRACT:Objective To investigate the effect of butyrylchitosan on the expression of proliferating cell nuclear antigen (PCNA) in fibroblast proliferation of rabbit eyes after filtering operation. Methods Twenty-four New Zealand rabbits were randomly divided into 2 groups, with 12 rabbits in each group. Rabbits in one group received butyrylchitosan under scleral patch of trabeculectomy in right eyes and trabeculectomy in left eyes (trabeculectomy group). Rabbits in the other group received mitomycin C (MMC) in trabeculectomy in right eyes (MMC group) and without operation in left eyes. Rabbits were killed 1, 4, and 12 weeks after operations. Immunohistochemical staining was used to detected PCNA expression in fibroblast. Results After use of butyrylchitosan, the PCNA expression significantly decreased compared with trabeculectomy group (P<0.001). PCNA expression in MMC group was significantly lower than in trabeculectomy group (P<0.001). Conclusion Using butyrylchitosan under scleral patch of trabeculectomy decreases PCNA expression in proli- ferating cell and inhibits the scarring at filtering site.

Key words: butyrylcditosan, proliferatinc cell nuclear anticen, trabeculectomy, scarrinc, claucoma