中国医学科学院学报

高级检索
中国医学科学院学报

中国医学科学院学报 ›› 2009, Vol. 31 ›› Issue (1): 20-23.doi: 10.3881/j.issn.1000-503X.2009.01.007

• 论著 • 上一篇    下一篇

结节病患者皮肤及淋巴结组织中分枝杆菌基因的检测和鉴定

丁晓岚;蔡林;张建中   

  1. 北京大学人民医院皮肤科,北京100044
  • 收稿日期:2008-12-04 修回日期:1900-01-01 出版日期:2009-02-28 发布日期:2009-02-28
  • 通讯作者: 张建中

Detection and Identification of Mycobacterial Gene in Skin Lesions and Lymph Nodes in Patients with Sarcoidosis

DING Xiao-lan;CAI Lin;ZHANG Jian-zhong   

  1. Department of Dermatology, Peking University People’s Hospital,Beijing 100044,China
  • Received:2008-12-04 Revised:1900-01-01 Online:2009-02-28 Published:2009-02-28
  • Contact: ZHANG Jian-zhong

摘要: 摘要:目的 探讨分枝杆菌在结节病发病中的作用。方法 采用巢式聚合酶链式反应-限制性片段长度多态性和PCR产物测序的方法,对31例福尔马林固定-石蜡包埋的结节病组织标本(皮肤19例,淋巴结12例)、14例正常皮肤石蜡标本和3例皮肤结核石蜡标本进行检测。结果 19例结节病皮肤标本中,有4例(21.1%)扩增出相对分子质量为65 000的分枝杆菌热休克蛋白基因,其中1例为结核分枝杆菌,2例为龟分枝杆菌,1例为戈登分枝杆菌。12例结节病淋巴结组织和14例正常皮肤组织标本中均未检测到分枝杆菌基因。3例皮肤结核标本中检测出结核分枝杆菌基因。结论 分枝杆菌可能与皮肤结节病发病有关。

关键词: 结节病, 分枝杆菌基因, 皮肤, 淋巴结

Abstract: ABSTRACT:Objective To investigate the possible role of mycobacteria in the pathogenesis of sarcoidosis. Methods We used nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) and gene sequencing method to examine 31 formalin-fixed, paraffin-embedded specimens (including 19 skin lesions and 12 lymph nodes ) obtained from patients with sarcoidosis, 14 normal skin specimens, and 3 cutaneous tuberculosis specimens. Results The 65kD mycobacterial heat shock protein gene was found in 4 out of 19 (21.1%) skin specimens of sarcoidosis. The mycobacteria included M. tuberculosis (n=1), M. chelonei (n=2), and M. gordonae (n=1). Mycobacterial DNA was negative in 12 lymph node specimens and 14 normal skin specimens. M.tuberculosis gene was detected in all 3 specimens of cutaneous tuberculosis. Conclusion Mycobacteria may play a role in the pathogenesis of cutaneous sarcoidosis.

Key words: sarcoidosis, mycobacteria cene, skin, lympd node