Application of Trichloroacetic Acid-Acetone Precipitation Method for Protein Extraction in Bone Tissue
CUI Yong-feng1, YANG Zhen2, ZHU Bao-hua1, XIAO Lu-wei2, TONG Pei-jian2*
1Department of Orthopedics, the First People's Hospital of Xiaoshan District, Hangzhou 311200, China 2Institute of Orthopedics, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China
Objective To explore the feasibility and efficiency of extracting protein from bone tissue using trichloroacetic acid (TCA)-acetone precipitation method. Methods Hydrochloric acid (HCL) decalcification method and TCA-acetone precipitation method were separately used for bone protein extraction. The efficiencies of these two methods were compared. Results TCA-acetone precipitation method had significantly higher extraction efficiency. Compared with HCL decalcification method, it had less pollution from bone marrow hematopoietic tissue. Protein band distribution was similar between these two methods. Conclusion TCA-acetone precipitation method is useful for bone proteomics research.